塔里木盆地5个生态小区稀有放线菌分离及合成抗生素基因分布

doi:10.13560/j.cnki.biotech.bull.1985.2018-0011

生物技术通报 ›› 2018, Vol. 34 ›› Issue (9): 230-236.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0011

塔里木盆地5个生态小区稀有放线菌分离及合成抗生素基因分布

朱荣贵¹, 关统伟², 姜秀娟¹

1. 四川理工学院生物工程学院,自贡 643000;
2. 西华大学食品与生物工程学院,成都 610039

收稿日期:2018-01-03 出版日期:2018-09-26 发布日期:2018-10-10
作者简介:朱荣贵,男,硕士研究生,研究方向：放线菌资源;E-mail：zrg07@sina.cn
基金资助:
国家 “973 计划” 前期研究专项（2010CB134505）,国家自然科学基金项目（31060001）,四川省教育厅项目（16ZB0244）,校级人才引进项目（2015RC04）

Isolation of Rare Actinobacteria in 5 Ecodistricts of Tarim Basin and Distribution of the Genes Synthesizing Antibiotics

ZHU Rong-gui¹, GUAN Tong-wei², JIANG Xiu-juan¹

1. College of Bioengineering,Sichuan University of Science Engineering,Zigong 643000;
2. College of Food Science and Biological Engineering,Xihua University,Chengdu 610039

Received:2018-01-03 Published:2018-09-26 Online:2018-10-10

摘要/Abstract

摘要： 为调查塔里木盆地5个代表性生态小区的稀有放线菌分布,并评估它们产生抗生素类活性物质的潜力。采集塔里木盆地5个生态小区混合土样5份,采用 7 种培养基分离样品中的稀有放线菌。通过检测分析I型PKS、II型PKS、NRPS、APH、HMG-CoA五种抗生素合成相关基因的分布,评估该地区稀有放线菌产生抗生素的潜力。结果表明：（1）基于分子鉴定,经合并重复,共分离得到18种稀有放线菌,属于放线菌的10个属。（2）塔里木河河岸林生态小区土样稀有放线菌分离种类最多（10种）,为8个属,塔克拉玛干沙漠塔里木河东部生态小区最少（4种）,为4个属。（3）18株稀有放线菌中有9株含有I型PKS基因、4株菌含有II型PKS基因、4株菌含有APH基因、3株菌含有NRPS基因,有一株链孢囊菌同时含有I型PKS、II型PKS、NRPS、APH 4种基因。5个生态小区稀有放线菌种类较多,并含有较为丰富的与抗生素合成相关的基因。

关键词: 塔里木盆地, 生态小区, 稀有放线菌, 功能基因筛选

Abstract: This work is for investigating the distribution of rare actinobacteria in different ecodistricts and assessing the potential of antibiotic production of these actinobacteria. Total 5 soil samples were collected from the Tarim Basin,and rare actinobacteria were isolated from these samples by 7 media. And then the potential of antibiotic productions was assessed by detecting type I PKS,type II PKS,NRPS,APH and HMG-CoA genes,related to antibiotics synthesis. The results showed that：（1）by molecular identification,we obtained 18 kinds of rare actinobacteria strains belonging to 10 different genera;（2）the riparian forest ecodistrict in the Tarim Basin had the most species of rare actinobacteria,and there were 8 genera;while the least one was the east of the Talimu river ecodistrict in Taklimakan desert,and there were 4 genera;（3）9 of these 18 strains had type I PKS gene,4 strains had type II PKS gene and APH gene,and 3 strains had NRPS gene;there was one Streptosporangium strain that contained type I PKS gene,type II PKS gene,NRPS gene and APH gene. Conclusively,5 ecological zones are found to have relatively more rare actinomycetes which also contain abundant genes in associated with the synthesis of antibiotics.

Key words: Tarim Basin, ecodistrict, rare actinobacteria, functional genes screening

朱荣贵, 关统伟, 姜秀娟. 塔里木盆地5个生态小区稀有放线菌分离及合成抗生素基因分布[J]. 生物技术通报, 2018, 34(9): 230-236.

ZHU Rong-gui, GUAN Tong-wei, JIANG Xiu-juan. Isolation of Rare Actinobacteria in 5 Ecodistricts of Tarim Basin and Distribution of the Genes Synthesizing Antibiotics[J]. Biotechnology Bulletin, 2018, 34(9): 230-236.

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塔里木盆地5个生态小区稀有放线菌分离及合成抗生素基因分布

Isolation of Rare Actinobacteria in 5 Ecodistricts of Tarim Basin and Distribution of the Genes Synthesizing Antibiotics

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