新疆阿勒泰冷水鱼屎肠球菌耐药性及耐药机理研究

doi:10.13560/j.cnki.biotech.bull.1985.2018-0339

生物技术通报 ›› 2018, Vol. 34 ›› Issue (10): 143-149.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0339

新疆阿勒泰冷水鱼屎肠球菌耐药性及耐药机理研究

肖何, 张艳, 王永瑞, 倪永清

新疆石河子大学食品学院,石河子 832000

收稿日期:2018-04-12 出版日期:2018-10-26 发布日期:2018-11-07
作者简介:肖何,硕士研究生,研究方向：食品微生物;E-mail：xiaohe200888@163.com
基金资助:
国家自然科学基金项目（31760446）

Antibiotic Resistance and Resistance Mechanism in Enterococcus faecium from Cold-water Fish in Aletai Prefecture of Xinjiang

XIAO He, ZHANG Yan, WANG Yong-rui, NI Yong-qing

Food College,Shihezi University,Shihezi 832000

Received:2018-04-12 Published:2018-10-26 Online:2018-11-07

摘要/Abstract

摘要： 对分离自新疆阿勒泰冷水鱼的屎肠球菌进行耐药性、耐药基因和毒力因子分析从而对冷水鱼的安全性进行评估。采用纸片法检测屎肠球菌对12种抗生素的敏感性及PCR检测相关耐药基因、毒力因子分布。13株分离自冷水鱼肠道的屎肠球菌对12种抗生素均未表现出耐药性,但是有11株对环丙沙星表现中介（中度敏感）,2株对链霉素表现中介（中度敏感）。13株屎肠球菌都携带环丙沙星耐药基因gyrA,其中有2株同时携带环丙沙星耐药基因parC。13株屎肠球菌中有1株菌携带四环素耐药基因tetK基因,1株菌仅携带tetL基因,4株同时携带tetM和tetL基因。13株屎肠球菌对毒力因子的检测结果均呈阴性。新疆冷水鱼肠道屎肠球菌的耐药及毒力因子的分析显示冷水鱼的消费对消费者并无安全隐患。

关键词: 冷水鱼, 屎肠球菌, 环丙沙星

Abstract: The antibiotic resistance,resistance genes and virulence factors of Enterococcus faecium strains from cold-water fish that collected from Aletai prefecture in Xinjiang were analyzed to evaluate the safety of cold-water fish. Disc diffusion method was used to detect the antimicrobial susceptibility of E. faecium strains to 12 antibiotics. Resistance genes and virulence factors were amplified by PCR. All the 13 E. faecium strains from intestine of cold-water fish were not resistant to the 12 antibiotics,while 11 strains being intermediate to ciprofloxacin and 2 strains being intermediate to streptomycin. All the 13 strains harbored the ciprofloxacin-resistant gene gyrA,while 2 strains carried the ciprofloxacin-resistant gene parC. Among the 13 strains,one strain carried achromycin-resistant gene tetK,one contained gene tetL,and 4 harbored the gene tetM and tetL. The 13 strains presented negative to the virulence factors. The analysis of antibiotic resistance and virulence factors of E. faecium strains suggests that the consumption of cold-water fish does not have a potential risk to the health of consumers.

Key words: cold-water fish, Enterococcus faecium, ciprofloxacin

肖何, 张艳, 王永瑞, 倪永清. 新疆阿勒泰冷水鱼屎肠球菌耐药性及耐药机理研究[J]. 生物技术通报, 2018, 34(10): 143-149.

XIAO He, ZHANG Yan, WANG Yong-rui, NI Yong-qing. Antibiotic Resistance and Resistance Mechanism in Enterococcus faecium from Cold-water Fish in Aletai Prefecture of Xinjiang[J]. Biotechnology Bulletin, 2018, 34(10): 143-149.

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新疆阿勒泰冷水鱼屎肠球菌耐药性及耐药机理研究

Antibiotic Resistance and Resistance Mechanism in Enterococcus faecium from Cold-water Fish in Aletai Prefecture of Xinjiang

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