玫瑰黄链霉菌NKZ-259发酵培养基的优化

doi:10.13560/j.cnki.biotech.bull.1985.2018-0761

摘要/Abstract

摘要： 玫瑰黄链霉菌NKZ-259是一株生防菌株,其次级代谢能产生植物生长调节类物质吲哚乙酸（IAA）。为了进一步提高菌株代谢产生IAA的含量,本试验对该菌株的发酵培养基进行了优化。利用单因子试验确定发酵培养基中最适的6种营养成分为葡萄糖、可溶性淀粉、蛋白胨、硝酸钾、磷酸氢二钾和L-色氨酸;通过Plackett-Burman设计筛选出影响菌株发酵产生IAA的主要因素为L-色氨酸、葡萄糖和磷酸氢二钾;采用中心组合试验（CCD）及响应面法分析各因素的交互作用。最终确定菌株代谢产生IAA的最优发酵培养基为：L-色氨酸2.24 g/L,葡萄糖20.7 g/L,磷酸氢二钾0.5 g/L,可溶性淀粉10 g/L,蛋白胨3 g/L,硝酸钾4.5 g/L;使用优化后的发酵培养基菌株代谢产生IAA的含量为45.377 4 μg/mL,比原始发酵培养基IAA的含量提高了3倍。

关键词: 吲哚-3-乙酸, 发酵培养基, 响应面法

Abstract: Streptomyces roseoflavus NKZ-259 is a biocontrol strain and plant growth regulator indole-3-acetic acid（IAA）is produced in the process of secondary metabolism. The fermentation medium was optimized in this study in order to increase the content of IAA produced by a strain. The 6 optimal nutrients of fermentation medium were determined by single factor experiment,and they were glucose,soluble starch,peptone,potassium nitrate,dipotassium hydrogen phosphate,and L-tryptophan,respectively. Plackett-Burman design method was applied to screen major factors affecting the fermentation production of IAA,which was L-tryptophan,glucose,and dipotassium hydrogen phosphate. Central combination design（CCD）and response surface methodology were used to analyze the interactions of all factors. Finally,the optimized fermentation medium was composed as follow：L-tryptophan 2.24 g/L,glucose 20.7 g/L,dipotassium hydrogen phosphate 0.5 g/L,soluble starch 10 g/L,peptone 3 g/L,potassium nitrate 4.5 g/L. The concentration of IAA reached 45.377 4 μg/mL after the medium optimized,which was increased 3 times compared to the original fermentation medium.

Key words: indole-3-acetic acid（IAA）, fermentation medium, response surface methodology

麻金金, 葛蓓孛, 施李鸣, 刘炳花, 韦秋合, 张克诚. 玫瑰黄链霉菌NKZ-259发酵培养基的优化[J]. 生物技术通报, 2019, 35(2): 85-92.

MA Jin-jin, GE Bei-bei, SHI Li-ming, LIU Bing-hua, WEI Qiu-he, ZHANG Ke-cheng. Optimization of Fermentation Medium for Streptomyces roseoflavus NKZ-259[J]. Biotechnology Bulletin, 2019, 35(2): 85-92.

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