海洋源乳酸菌AI-2类群体感应抑制剂对单增李斯特菌抑制效果研究

doi:10.13560/j.cnki.biotech.bull.1985.2018-0934

摘要/Abstract

摘要： 单增李斯特菌（Listeria monocytogenes）AI-2类群体感应系统调控的生物被膜与毒力因子是导致其高致病率与高死亡率的主要原因。以测定报告菌哈维氏弧菌BB170的发光值作为筛选指标,对16株海洋源乳酸菌代谢产物中筛选L. m AI-2信号分子的群体感应抑制剂（QSIs）,并通过测检QSIs对L. m的MIC值、生长曲线、动力形成、生物被膜形成量,评价QSIs对L. m的控制效应。结果表明：从16株海洋源乳酸菌中筛选到6株乳酸菌的乙酸乙酯提取物对L. m AI-2信号分子活性有良好的抑制作用,占筛选菌株的37.5%,且抑制率均达75%以上,其中菌株Pediococcus pentosaceus zy-B-1乙酸乙酯提取物QSI-B-1抑制率最高（98.5%）,并对L. m作用的MIC值为250 μg/mL,随着QSI-B-1浓度的增加,对L. m菌落生长与动力形成的抑制作用逐渐加强,形成的生物被膜结构愈加疏松。本研究为利用从海洋环境中筛选乳酸菌源L. m QSIs提供依据。

关键词: 海洋源乳酸菌, 单增李斯特菌, 群体感应抑制剂, AI-2

Abstract: The biofilm and virulence factors regulated by AI-2（Autoinducer 2）quorum sensing system are the main causes of high morbidity and mortality of Listeria monocytogenes. In this experiment,the luminescence value of the reporter Vibrio harveyi BB170 was used as the index to screen the AI-2 quorum sensing inhibitors（QSIs）of L. monocytogene from the metabolites of 16 marine lactic acid bacteria. The control effect of QSIs on L. m was evaluated by measuring its MIC value,growth curve,dynamic formation,and biofilm formation. The results showed that the ethyl acetate extracts from 6 strains of 16 marine lactic acid bacteria had a promising inhibitory effect on the AI-2 signaling molecule activity of L. monocytogene,accounting for 37.5% of the screened strains,and the inhibition rate reached over 75 %. Among them,the ethyl acetate extracts from strain Pediococcus pentosaceus zy-B-1 presented the highest inhibition rate（98.5%）and the MIC value to L. monocytogene was 250 μg/mL. As the concentration of QSI-B-1 increased,the inhibitory effect on the growth and dynamic formation of L. monocytogene was gradually strengthened,and the biofilm structure became more and more loose. This study provides a basis for screening the QSIs of L. monocytogene from the marine lactic acid bacteria.

Key words: marine lactic acid bacteria, Listeria monocytogenes, quorum sensing inhibitor, AI-2

黄湘湄, 吴雅茜, 刘颖, 梁嘉烨, 苏伟明. 海洋源乳酸菌AI-2类群体感应抑制剂对单增李斯特菌抑制效果研究[J]. 生物技术通报, 2019, 35(4): 36-42.

HUANG Xiang-mei, WU Ya-qian, LIU Ying, LIANG Jia-ye, SU Wei-ming. Inhibitory Effects of AI-2 Quorum Sensing Inhibitors from Marine Lactic Acid Bacteria on Listeria monocytogenes[J]. Biotechnology Bulletin, 2019, 35(4): 36-42.

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