生物技术通报 ›› 2019, Vol. 35 ›› Issue (4): 82-87.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0888

• 研究报告 • 上一篇    下一篇

母牦牛生殖系统中低氧诱导因子基因的表达分析

何向东, 夏忆, 吉格莫体, 王会, 字向东   

  1. 西南民族大学动物科学国家民委重点实验室,成都 610041
  • 收稿日期:2018-10-17 出版日期:2019-04-26 发布日期:2019-05-05
  • 作者简介:何向东,男,硕士研究生,研究方向:遗传学;E-mail:1358964559@qq.com
  • 基金资助:
    国家重点研发计划项目(2018YFD0502303)

mRNA Expressions of Hypoxia Inducible Factor Genes in Female Yak Reproductive System

HE Xiang-dong, XIA Yi, JIGE Mo-ti, WANG Hui, ZI Xiang-dong   

  1. Key Laboratory of Animal Science of State Ethnic Affairs Commission,Southwest Minzu University,Chengdu 610041
  • Received:2018-10-17 Published:2019-04-26 Online:2019-05-05

摘要: 低氧诱导因子1α(Hypoxia-inducible factor 1α,HIF-1α)和2α(Hypoxia-inducible factor 2α,HIF-2α)是诱导低氧基因和修复细胞氧内环境的核心调节因子,为了研究生活在青藏高原牦牛的繁殖机能对低氧环境的适应机制,采集卵泡期的5头成年母牦牛和母黄牛的下丘脑、脑垂体、卵巢、输卵管和子宫组织,利用RT-qPCR技术检测HIF-1α和HIF-2α mRNA的表达量。结果表明:HIF-1α mRNA在牦牛输卵管中表达量最高,牦牛脑垂体和输卵管表达量极显著高于黄牛(P<0.01)。HIF-2α mRNA在牦牛脑垂体表达量极显著高于黄牛(P<0.01),在输卵管表达量显著高于黄牛(P<0.05)。说明在低氧环境条件下,母牦牛可能通过调节生殖生殖系统中HIF-1α和HIF-2α基因的表达维持其繁殖机能。

关键词: 牦牛, 黄牛, HIF-1α, HIF-2α, 组织表达

Abstract: Hypoxia-inducible factor 1α(HIF-1α)and 2α(HIF-2α)are core regulators for inducing hypoxia genes and repairing cellular oxygen environment. This study aims to investigate the adaptation mechanism of the reproduction of yak living on the Qinghai-Tibet Plateau to the hypoxic environment. Hypothalamus,pituitary,oviduct,ovary and uterus tissue samples were collected from five adult and health female yaks and cattle during the follicular phase,respectively. Tissue expression characteristics of these genes were performed by RT-qPCR. The results showed that the mRNA expression level of HIF-1α in the oviduct was the highest,and was very significantly higher in the pituitary and oviduct in yak than in cattle(P<0.01). Meanwhile,the mRNA expression of HIF-2α was very significantly higher in the hypothalamus(P<0.01)and significantly higher in the ovary of yak than in cattle(P<0.05). These results suggest that female yaks maintain their reproductive function mainly by regulating HIF-1α and HIF-2α genes in the reproductive system under hypoxic conditions.

Key words: yak, cattle, HIF-1α, HIF-2α, tissue expression