关键词: 黑果枸杞, 种源, 组织培养, 生根, 分化

Abstract:

This work aims to establish an efficient and stable tissue culture regeneration system suitable for different provenances and multiple genotypes of Lycium ruthenicum Murr. and lay a foundation for planting-raising and large-scale popularization. The seeds of L. ruthenicum from 7 different provenances were collected and sterile seedlings were prepared. Plant tissue culture techniques were used to study the effects of different kinds and concentrations of plant growth regulators on the rooting and differentiation of L. ruthenicum. In the process of screening stem segment differentiation medium of L. ruthenicum,aiming at the vitrification phenomenon which is easy to appear in the culture process of L. ruthenicum,a medium suitable for the stem differentiations of multi-provenance and multi-genotype L. ruthenicum was determined through comparison of various media as such：MS + sucrose 30 g/L + agar 7 g/L + 6-BA 0.1 mg/L. A medium suitable for the leaf differentiation of multi-genotype L. ruthenicum was obtained as MS + sucrose 30 g/L + agar 6 g/L + NAA 0.5 mg/L + 6-BA 0.5 mg/L by adjusting the concentration of auxin and cell division while screening leaf-induced differentiation medium of L. ruthenicum. An optimal medium 1/2 MS + sucrose 30 g/L+ agar 7.5 g/L + IBA0.25 mg/L for the rooting of and multi-provenance and multi-genotype L. ruthenicum was determined by changing the concentration of auxin. Based on the large-scale experiments,the problems of vitrification,non-rooting,non-germination,etc.,which often occur during rooting and differentiation of Lycium ruthenicum from different provenances when using one medium,were overcome. In another word,an efficient and stable tissue culture and regeneration system suitable for multi-provenance and multi-genotype L. ruthenicum was established.

Key words: Lycium ruthenicum Murr., provenance, tissue culture, rooting, differentiation