关键词: ATP合成, 腺苷激酶, 腺苷酸激酶, 乙酸激酶, S-腺苷甲硫氨酸

Abstract: In order to achieve ATP production as well as reduce the cost of producing S-adenosylmethionine,a recombinant Escherichia coli strain expressing adenosine kinase,adenylate kinase and acetate kinase simultaneously was constructed for ATP synthesis. The conditions of transforming ATP were optimized,and the optimized reaction system was as：adenosine 30 mmol/L,acetylphosphate dilithium 135 mmol/L,magnesium sulfate 5 mmol/L,borax 50 mmol/L,E. coli strain 2 g/L（wet weight）,initial pH 7.5,the reaction temperature 35℃,and reacting for 3 h,the conversion rate was over 99%. Based on the above result,the reaction system was enlarged in 5 L. After reaction ended,50 g/L（wet weight）recombinant E. coli strain expressing adenosylmethionine synthase,65 mmol/L D,L-methionine and 15 mmol/L magnesium sulfate were added to the reaction system,the concentration of S-adenosylmethionine was up to 8.7 g/L and the conversion rate reached 72.5% after 18 h transformation.

Key words: ATP synthesis, adenosine kinase, adenylate kinase, acetate kinase, S-adenosylmethionine