一种三联乳酸菌的体外降胆固醇和抗氧化能力研究

doi:10.13560/j.cnki.biotech.bull.1985.2020-0399

摘要/Abstract

摘要：

为获得一种易在体内定植且降胆固醇和抗氧化能力较强三联乳酸菌,以干酪乳杆菌（Lactobacillus casei S1）、屎肠球菌（Enterococcus faecium S4）和哈伯氏乳杆菌（Lactobacillus harbinensis S6）3株供试菌株制备三联乳酸菌,考察三联乳酸菌的人工胃肠耐受性、降胆固醇能力、γ-氨基丁酸（Gamma-aminobutyric acid,GABA）含量和抗氧化活性。结果表明,三联乳酸菌在人工胃肠液处理24 h后,存活率为47.88%,活菌数为1.25×10⁹CFU/mL;总胆固醇去除率、胆盐水解酶（Bile salt hydrolase,BSH）比活以及GABA含量分别为73.43%、151.40 U/mg、1.387 mg/mL;还原力、超氧自由基清除率、羟自由基清除率分别为1.52、95.68%、89.93%,显著高于对照组（P<0.01）。三联乳酸菌的人工胃肠液耐受较强,且具有较强的降胆固醇和抗氧化能力,可进一步开发为功能性微生态制剂或保健品。

关键词: 三联乳酸菌, 人工胃肠液耐受性, 降胆固醇, γ-氨基丁酸;, 抗氧化活性

Abstract:

In order to obtain a tri-lactobacillus that is easy to colonize in vivo and has strong ability of lowering cholesterol and anti-oxidation,three strains of Lactobacillus casei S1,Enterococcus faecium S4 and Lactobacillus harbinensis S6 were used to form tri-lactobacillus. The artificial gastrointestinal tolerance,cholesterol-lowering ability,yield of γ-aminobutyric acid（GABA）,and antioxidant activity of tri-lactobacillus were investigated. The results showed that the survival rate of tri-lactobacillus was 47.88% and the number of viable bacteria was 1.25×10⁹CFU/mL after 24 h of treatment with artificial gastric intestinal fluid. The total cholesterol removal rate,specific activity of bile salt hydrolase and GABA yield were 73.43%,151.40 U/mg and 1.387 mg/mL,respectively;and the reducing power,superoxide radical clearance rate and hydroxyl radical clearance were 1.52,95.68% and 89.93%,respectively. It was significantly higher than the control group（P < 0.01）. The tolerance of tri-lactobacillus to artificial gastric and intestinal fluid is strong,and it has strong ability of lowering cholesterol and anti-oxidation,thus it can be further developed as functional microecological preparation or health care product.

Key words: tri-lactobacillus, tolerance of artificial gastric intestinal fluid, cholesterol lowering, γ-aminobutyric acid;, antioxidant activity

黄元霞, 彭传海, 丁宁, 邱忠平, 李星, 邹美慧. 一种三联乳酸菌的体外降胆固醇和抗氧化能力研究[J]. 生物技术通报, 2020, 36(12): 113-120.

HUANG Yuan-xia, PENG Chuan-hai, DING Ning, QIU Zhong-ping, LI Xing, ZOU Mei-hui. Study on the Cholesterol-lowering and Antioxidant Abilities of a Tri-lactobacillus In Vitro[J]. Biotechnology Bulletin, 2020, 36(12): 113-120.

图/表 7

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	胆固醇去除率/%	BSH比活/（U·mg^-1）
三联乳酸菌	73.43±1.22a	151.40±2.82a
对照组	58.21±2.09b	98.77±0.28b

	胆固醇去除率/%	BSH比活/（U·mg^-1）
三联乳酸菌	73.43±1.22a	151.40±2.82a
对照组	58.21±2.09b	98.77±0.28b

去除胆固醇分布	三联乳酸菌
与胆盐共沉淀/（μg·mL^-1）	26.43±0.56
共沉淀占总去除量的比例/%	56.46±0.68
菌体吸收/（μg·mL^-1）	15.54±0.41
菌体吸收占总去除量的比例/%	39.62±0.58
细胞膜结合/（μg·mL^-1）	1.83±0.07
细胞膜结合占总去除量的比例/%	3.90±0.13

去除胆固醇分布	三联乳酸菌
与胆盐共沉淀/（μg·mL^-1）	26.43±0.56
共沉淀占总去除量的比例/%	56.46±0.68
菌体吸收/（μg·mL^-1）	15.54±0.41
菌体吸收占总去除量的比例/%	39.62±0.58
细胞膜结合/（μg·mL^-1）	1.83±0.07
细胞膜结合占总去除量的比例/%	3.90±0.13

相关系数r	Y （酶比活）	X₁ （共沉淀）	X₂ （菌体吸收）	X₃ （细胞膜结合）
Y（酶比活）	1
X₁（共沉淀）	0.898^**	1
X₂（菌体吸收）	-0.528^*	-0.265	1
X₃（细胞膜结合）	-0.033	0.059	0.631^*	1