海南山栏稻抗旱基因转录组分析

doi:10.13560/j.cnki.biotech.bull.1985.2020-0422

摘要/Abstract

摘要：

山栏稻是海南独有的地方旱稻,具有丰富的抗旱基因资源。本研究以海南山栏稻品种白沙糯为材料,利用转录组测序技术研究在模拟干旱胁迫后其转录组水平的变化。主要结果如下：山栏稻白沙糯干旱胁迫后有2 791个基因差异表达,差异表达基因中有184个转录因子在干旱胁迫后显著上调,包含了Znf、AP2/ERF、MYB、NAC、bZIP等抗旱转录因子,其中Znf家族转录因子所占数量最多。表达分析显示一些未注释的基因,如Os01g0910800随着胁迫时间的延长,表达水平逐渐上升且倍数明显。该研究获得的转录组数据和数字基因表达谱,为山栏稻抗旱机理的研究提供了信息资源,并为进一步解析山栏稻的抗旱机理奠定基础。

关键词: 山栏稻, 旱稻, 转录组测序, 抗旱基因

Abstract:

Shanlan upland rice is an unique local upland rice in Hainan province,which is rich in drought- resistant gene resources. In this study,a Hainan Shanlan upland rice variety,Baishanuo was used to study the changes of transcriptome level after simulated drought stress. The main results were as follows：2 791 genes were differentially expressed after simulated drought stress,and 184 transcription factors were significantly up-regulated after simulated drought stress. These transcription factors include Znf,AP2/ERF,MYB,NAC,bZIP and so on,among which the number of Znf transcription factors was the largest. Expression analysis showed that some unannotated genes,such as Os01g0910800,their expressions increased with the time of stress,and the multiple of expression was obvious. The transcriptome data and digital gene expression profiling data obtained in this study provide information for the study of drought resistance mechanism of Shanlan upland rice,and lay a foundation for further analysis of the drought resistance mechanism of Shanlan upland rice.

Key words: Shanlan upland rice, upland rice, RNA-seq, drought resistance gene

翟楠鑫, 迟会, 夏玥琳, 刘彩月, 裴新梧, 袁潜华. 海南山栏稻抗旱基因转录组分析[J]. 生物技术通报, 2020, 36(12): 12-20.

ZHAI Nan-xin, CHI Hui, XIA Yue-lin, LIU Cai-yue, PEI Xin-wu, YUAN Qian-hua. Transcriptome Analysis of Drought-resistant Genes in Hainan Shanlan Upland Rice[J]. Biotechnology Bulletin, 2020, 36(12): 12-20.

图/表 10

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Gene_ID /Name	Forward primer（5'-3'）	Reverse primer（5'-3'）
OsLEA3-2	CCAAGAACAAGCTGGGCGAG	CTTGAACTCCGTCGCCTTCT
RAB21	GCTCCAGCTCAAGCTCGTC	CATGGCATGCTGCTGCTC
Os09g0351700	TCTCGAACAGCAACCTGCAT	GGATGGAATTGTCCCGCTCA
Os07g0658600	GGGTTCGACACGTGCTACA	GGTGTGGATCACCACGTTCT
Os02g0693700	GTTCTACGAGCCTCTCACGG	CTGGTTGACGAGGCCAATCT
Os11g0115100	TCAGGAACCTCAACATGGGC	CTTGGAGCAGTCGACGGAG
Os07g0251900	CTTCGCTCGGGAACCTAACA	TGAGGTAGAGTGACCGGAGG
Os01g0910800	TGGCGCAGTACTACTCGGA	GCCGGTGCAGGAGTCGTA
OsActin	TGGCATCTCTCAGCACATTCC	TGCACAATGGATGGGCCAGA

Gene_ID /Name	Forward primer（5'-3'）	Reverse primer（5'-3'）
OsLEA3-2	CCAAGAACAAGCTGGGCGAG	CTTGAACTCCGTCGCCTTCT
RAB21	GCTCCAGCTCAAGCTCGTC	CATGGCATGCTGCTGCTC
Os09g0351700	TCTCGAACAGCAACCTGCAT	GGATGGAATTGTCCCGCTCA
Os07g0658600	GGGTTCGACACGTGCTACA	GGTGTGGATCACCACGTTCT
Os02g0693700	GTTCTACGAGCCTCTCACGG	CTGGTTGACGAGGCCAATCT
Os11g0115100	TCAGGAACCTCAACATGGGC	CTTGGAGCAGTCGACGGAG
Os07g0251900	CTTCGCTCGGGAACCTAACA	TGAGGTAGAGTGACCGGAGG
Os01g0910800	TGGCGCAGTACTACTCGGA	GCCGGTGCAGGAGTCGTA
OsActin	TGGCATCTCTCAGCACATTCC	TGCACAATGGATGGGCCAGA

Sample name	Total reads	Total mapped	Multiple mapped	Uniquely mapped	Non-splice reads	Splice reads
No-drought	57 202 689	53 682 100 93.77%	1 407 400 2.45%	52 274 700 91.32%	33 494 592 58.51%	18 780 108 32.81%
Drought	51 734 760	48 915 870 94.56%	1 027 373 1.99%	47 888 497 92.57%	30 689 990 59.31%	17 198 507 33.27%

Sample name	Total reads	Total mapped	Multiple mapped	Uniquely mapped	Non-splice reads	Splice reads
No-drought	57 202 689	53 682 100 93.77%	1 407 400 2.45%	52 274 700 91.32%	33 494 592 58.51%	18 780 108 32.81%
Drought	51 734 760	48 915 870 94.56%	1 027 373 1.99%	47 888 497 92.57%	30 689 990 59.31%	17 198 507 33.27%

Baishanuo upland rice	Up	Down	Total
Known genes	1 586	1 064	2 650
Unknown Gene	83	58	141
Total	1 669	1 122	2 791