皮克林乳液酶反应体系的构建与应用研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2020-0792

摘要/Abstract

摘要：

皮克林乳液是依靠固体颗粒稳定的乳液,具有抗聚结性、稳定性好,颗粒易于分离、成本低、毒性小等优点。研究表明将其应用于多相酶催化反应中,可以大大提高体系中的界面反应面积,加快传质速率。综述了皮克林乳液常用的稳定剂颗粒以及颗粒表面改性对乳液稳定的影响。在此基础上,进一步详细论述了皮克林乳液在酶反应体系中应用以及具有“开关”调控的智能皮克林乳液酶反应体系,并对皮克林乳液酶反应体系未来的发展进行了展望。旨在为构建更高效、绿色的酶催化反应提供更多的思路。

关键词: 皮克林乳液, 固体颗粒, 酶催化, 固定化酶, 智能调控

Abstract:

Pickering emulsion is stabilized by solid particles and possesses the advantages of anti-agglomeration,good stability,easy separation of particles,low cost and low toxicity. Studies have shown that its application in heterogeneous enzyme-catalyzed reactions may greatly increase the interface reaction area and accelerate the mass transfer rate. This article reviews the solid particles commonly used to stabilize Pickering emulsions and the effect of particle surface modification on emulsion stability. On this basis,the article further discusses the application of Pickering emulsion in the enzyme reaction system and the smart Pickering emulsion enzyme reaction system with “switch” regulation in detail,and prospects the development of Pickering emulsion enzyme catalytic reaction system. It aims to provide more ideas for constructing a more efficient and green enzyme-catalyzed reaction.

Key words: Pickering emulsion, solid particles, enzyme-catalyzed, immobilized enzyme, intelligent regulation

徐刘佳, 郑明明. 皮克林乳液酶反应体系的构建与应用研究进展[J]. 生物技术通报, 2021, 37(2): 187-194.

XU Liu-jia, ZHENG Ming-ming. Research Progress on the Construction and Application of Pickering Emulsion Enzymatic Reaction System[J]. Biotechnology Bulletin, 2021, 37(2): 187-194.

图/表 3

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颗粒种类	改性前		改性后
颗粒种类	直径/μm	三相接触角/°	直径/μm	三相接触角/°
淀粉球晶	3.97	39.5	3.88	74.4
大米淀粉	7.61	42.5	7.55	67.6
玉米淀粉	14.98	33.6	14.38	60.7
马铃薯淀粉	26.93	46.1	24.77	54.4

颗粒种类	改性前		改性后
颗粒种类	直径/μm	三相接触角/°	直径/μm	三相接触角/°
淀粉球晶	3.97	39.5	3.88	74.4
大米淀粉	7.61	42.5	7.55	67.6
玉米淀粉	14.98	33.6	14.38	60.7
马铃薯淀粉	26.93	46.1	24.77	54.4

酶的种类	稳定剂/载体	酶促反应	有机溶剂	转化率	参考文献
脂肪酶AYS	介孔碳球	植物甾醇和α-亚麻酸的酯化反应	-	1.5 h,92%	[22]
脂肪酶CALB	纳米聚合体PEG-b-P（S-co-TMI）	1-己醇与己酸的酯化反应	甲苯	24 h,80%	[38]
脂肪酶	海藻酸盐复合粒子E@Alg@s-TiO₂	1-己醇与己酸的酯化反应	正己烷	4 h,85-95%	[39]
脂肪酶CALB	纳米共聚物P（St-co-GMA）	己醇和己酸的酯化反应	庚烷	24 h,96.5%	[40]
脂肪酶CALB	二氧化硅纳米花	甲醇和废弃油生产生物柴油	环己烷	8.11 h,98.5%	[41]
脂肪酶CALB	功能化多壁碳纳米管MWCNTs-NH₂	马齿苋种子油和甲醇生产生物柴油	正庚烷	11.06 h,95.2%	[42]
脂肪酶CALB	介孔纳米二氧化硅	1-己醇与己酸的酯化反应	甲苯	20 min,88%	[43]

酶的种类	稳定剂/载体	酶促反应	有机溶剂	转化率	参考文献
脂肪酶AYS	介孔碳球	植物甾醇和α-亚麻酸的酯化反应	-	1.5 h,92%	[22]
脂肪酶CALB	纳米聚合体PEG-b-P（S-co-TMI）	1-己醇与己酸的酯化反应	甲苯	24 h,80%	[38]
脂肪酶	海藻酸盐复合粒子E@Alg@s-TiO₂	1-己醇与己酸的酯化反应	正己烷	4 h,85-95%	[39]
脂肪酶CALB	纳米共聚物P（St-co-GMA）	己醇和己酸的酯化反应	庚烷	24 h,96.5%	[40]
脂肪酶CALB	二氧化硅纳米花	甲醇和废弃油生产生物柴油	环己烷	8.11 h,98.5%	[41]
脂肪酶CALB	功能化多壁碳纳米管MWCNTs-NH₂	马齿苋种子油和甲醇生产生物柴油	正庚烷	11.06 h,95.2%	[42]
脂肪酶CALB	介孔纳米二氧化硅	1-己醇与己酸的酯化反应	甲苯	20 min,88%	[43]