生物技术通报 ›› 2021, Vol. 37 ›› Issue (10): 245-256.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1491

• 技术与方法 • 上一篇    下一篇

单细胞测序技术及其在毛囊发育中的应用

叶娜1,2(), 张晓兰2, 包鹏甲2, 王兴东1,2, 阎萍2(), 潘和平1()   

  1. 1.西北民族大学生命科学与工程学院,兰州 730030
    2.中国农业科学院兰州畜牧与兽药研究所 甘肃省牦牛繁育工程重点实验室,兰州 730050
  • 收稿日期:2020-12-09 出版日期:2021-10-26 发布日期:2021-11-12
  • 作者简介:叶娜,女,硕士研究生,研究方向:动物遗传育种与繁殖;E-mail: 1573030432@qq.com
  • 基金资助:
    国家自然科学基金地区科学基金项目(31660636)

Single Cell Sequencing Technology and Its Application in Hair Follicle Development

YE Na1,2(), ZHANG Xiao-lan2, BAO Peng-jia2, WANG Xing-dong1,2, YAN Ping2(), PAN He-ping1()   

  1. 1. College of Life Science and Engineering,Northwest University for Nationalities,Lanzhou 730030
    2. Key Laboratory of Yak Breeding Engineering of Gansu Province,Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050
  • Received:2020-12-09 Published:2021-10-26 Online:2021-11-12

摘要:

毛囊是一种具有独特结构的、呈周期性生长的微型器官,其形态发生始于胚胎期,由表皮上皮、间质真皮及特殊衍生物经过一系列相互作用诱导形成。出生后个体的毛囊开始进行周期性循环,包括生长期、退行期和休止期。单细胞转录组测序(single-cell RNA sequence,scRNA-seq)是一种新的测序方法,通过制备单细胞悬液或细胞群,利用二代测序(next generation sequencing)来识别单个细胞的基因表达信息,主要用于分析细胞间遗传和基因表达水平的差异,更好地了解单个细胞在微环境中的具体作用。通过单细胞转录组测序可以揭示复杂和稀有细胞群体以及基因之间的调节关系,跟踪不同细胞谱系在发育过程中的轨迹。本文就单细胞转录组测序技术及其在毛囊发育调控研究中的应用展开叙述,以期为揭示毛囊发育的分子调控机制研究提供理论参考。

关键词: 单细胞转录组测序, 单细胞分离技术, 毛囊发育

Abstract:

Hair follicle is a kind of micro-organ with unique structure and periodic growth,its morphogenesis begins in the embryonic stage and is induced by a series of interactions among epidermis epithelium,interstitial dermis and special derivatives. After birth,individual hair follicles begin to cycle periodically,including growth period,retrogression period and resting period. Single-cell RNA sequence(scRNA-seq)is a new sequencing method,mainly through the preparation of single cell suspension or cell groups and using the next generation sequencing to identify the gene expression information of the single cell. It is mainly used to analyze the differences of genetic and gene expression levels among cells,to better understand the specific role of a single cell in the microenvironment. The regulatory relationship between complex and rare cell populations and genes may be revealed via scRNA-seq,and the developmental trajectories of different cell lineages can be traced. This paper describes the scRNA-seq and its application in the regulation of hair follicle development,aiming to provide theoretical reference for revealing the molecular regulation mechanism of hair follicle development.

Key words: single-cell RNA sequencing, single cell isolation technique, hair follicle development