生物技术通报 ›› 2022, Vol. 38 ›› Issue (7): 171-177.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1294

• 研究报告 • 上一篇    下一篇

高粱双粒突变体Dgs的遗传分析与基因定位

周诗晨1(), 仪治本2, 王馨翊1, 杨晓颖1, 孙丽娜1, 栾维江1(), 梁闪闪1()   

  1. 1.天津师范大学生命科学学院 天津师范大学天津市动植物抗性重点实验室,天津 300387
    2.中北大学化学工程与技术学院,太原 030051
  • 收稿日期:2021-10-13 出版日期:2022-07-26 发布日期:2022-08-09
  • 作者简介:周诗晨,女,硕士研究生,研究方向:植物遗传学;E-mail: zschena@163.com
  • 基金资助:
    国家自然科学基金青年项目(32001513)

Genetic Analysis and Gene Mapping of Sorghum Double-grain Mutant Dgs

ZHOU Shi-chen1(), YI Zhi-ben2, WANG Xin-yi1, YANG Xiao-ying1, SUN Li-na1, LUAN Wei-jiang1(), LIANG Shan-shan1()   

  1. 1. College of Life Sciences,Tianjin Normal University/Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387
    2. School of Chemical Engineering and Technology,North University of China,Taiyuan 030051
  • Received:2021-10-13 Published:2022-07-26 Online:2022-08-09

摘要:

穗粒数对作物产量至关重要,对高粱双粒突变体基因进行定位,为下一步的基因克隆和功能分析奠定基础,为高粱产量性状的调控和解析提供依据。利用体视镜观察野生型和双粒突变体花器官表型,通过构建单粒材料晋粱5号和双粒突变体杂交的F2群体,分析突变体的遗传规律,采用BSA-seq测序与图位克隆相结合的方法进行连锁分析及基因定位。结果表明,与野生型相比,双粒突变体雌蕊和雄蕊数目增加,最终结一壳双粒;遗传分析表明双粒性状受1对显性基因控制;基因定位显示目的基因位于高粱第6染色体Indel2930和SSR7060标记之间,物理距离为404 kb。Dgs是一个全新的高粱双粒突变基因。

关键词: 高粱, 双粒突变体, 花器官, 基因定位, 遗传分析

Abstract:

Grain number per panicle is very important for crop yield. In order to identify and clone the gene controlling grain number per panicle,a double-grain mutant gene of sorghum[Sorghum bicolor(L.)Moench]was located,for laying a foundation for gene cloning and functional analysis and providing evidences for the regulation and analysis of sorghum yield traits. The flower organ phenotypes of wild-type sorghum plant and double-grain mutant were observed by stereoscopy. A F2 population was constructed from the cross between S. bicolor Jin 5 and the double-grain mutant to analyze the inheritance pattern of the mutant and gene mapping. BSA-seq sequencing and map cloning were used for linkage analysis and gene mapping. The results showed that,compared with wild type,the mutant mainly showed an increase in the number of pistil and stamen,ultimately two kernels in one pair of glumes. Genetic analysis indicated that the double-grain trait was controlled by a pair of dominant genes. Gene mapping showed that the target gene was located between markers Indel2930 and SSR7060 on the chromosome 6 of S. bicolor with physical distance of 404 kb. Dgs is a novel sorghum double-grain mutant gene.

Key words: sorghum, double-grain mutant, flower organ, gene mapping, genetic analycis