微流控技术在病原微生物检测中的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2023-0479

生物技术通报 ›› 2023, Vol. 39 ›› Issue (10): 107-114.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0479

微流控技术在病原微生物检测中的研究进展

万其武¹^,²^,⁴(), 包旭东¹^,², 丁柯¹^,³, 牟华明⁴(), 罗阳¹()

1.重庆大学医学院智慧检验与分子医学中心，重庆 400044
2.重庆大学生物工程学院，重庆 400044
3.重庆大学附属江津医院科教外事部，重庆 402260
4.重庆大学附属三峡医院心血管内科，重庆 400044

收稿日期:2023-05-18 出版日期:2023-10-26 发布日期:2023-11-28
通讯作者: 罗阳，男，博士，教授，研究方向：疾病早期检测、分子诊断；E-mail: luoy@cqu.edu.cn；
牟华明，男，博士，主任医师，研究方向：内科学（心血管病学）；E-mail: mouhm2002@aliyun.com
作者简介:万其武，男，硕士研究生，研究方向：疾病早期检测、分子诊断；E-mail: qiwuwan@163.com
基金资助:
国家杰出青年科学基金资助项目(82125022);重庆大学附属江津医院博士后科研启动项目(2022BHQDXM01);重庆市博士后科学基金项目(CSTB2022NSCQ-BHX0703)

Research Progress in Microfluidic Technology in the Detection of Pathogenic Microorganisms

WAN Qi-wu¹^,²^,⁴(), BAO Xu-dong¹^,², DING Ke¹^,³, MOU Hua-ming⁴(), LUO Yang¹()

1. Center of Smart Laboratory and Molecular Medicine, School of Medicine, Chongqing University, Chongqing 400044
2. College of Bioengineering，Chongqing University, Chongqing 400044
3. Foreign Affairs Department of Science and Technology, Jiangjin Hospital，Chongqing University, Chongqing 402260
4. Cardiovascular Medicine Department，Sanxia Hospital of Chongqing University, Chongqing 400044

Received:2023-05-18 Published:2023-10-26 Online:2023-11-28

摘要/Abstract

摘要：

快速、准确地检测病原微生物对于疫情防控和保障人民生命健康具有重大意义。近几年，研究者们通过合理地设计微流控芯片，将微流控技术与各种检测技术相结合，已经开发出了多种用于病原微生物检测的技术方法。相较于传统的病原微生物检测技术，微流控检测技术优势突出，具有操作人员技术要求不高、样本需求量少和自动化程度高等优点，适用于各种复杂环境下病原微生物的精准、快速检测。本文对微流控技术在病毒、细菌、真菌、衣原体及支原体等病原微生物的检测应用进行了综述，以期为病原微生物的检测提供研究思路，促进微流控技术在病原微生物检测中的发展，提高对疾病的预防和控制能力。

关键词: 病原微生物, 芯片, 微流控, 自动化

Abstract:

Rapid and accurate detection of pathogenic microorganisms is of great significance for epidemic prevention and control as well as the protection of people's lives and health. In recent years, researchers have developed a variety of technical methods for the detection of pathogenic microorganisms by reasonably designing microfluidic chips and combining microfluidic technology with various detection technologies. Compared with traditional pathogenic microorganism detection technology, microfluidic detection technology has outstanding advantages, i.e., low technical requirements for operators, less sample demand and high degree of automation, and is suitable for accurate and rapid detection of pathogenic microorganisms in various complex environments. In this paper, the application of microfluidic technology in the detection of pathogenic microorganisms such as viruses, bacteria, fungi, chlamydia and mycoplasma was reviewed, aiming to provide research ideas for the detection of pathogenic microorganisms, promote the development of microfluidic technology in the detection of pathogenic microorganisms, and improve the prevention and control ability of diseases.

Key words: pathogenic microorganisms, chip, microfluidics, automation

万其武, 包旭东, 丁柯, 牟华明, 罗阳. 微流控技术在病原微生物检测中的研究进展[J]. 生物技术通报, 2023, 39(10): 107-114.

WAN Qi-wu, BAO Xu-dong, DING Ke, MOU Hua-ming, LUO Yang. Research Progress in Microfluidic Technology in the Detection of Pathogenic Microorganisms[J]. Biotechnology Bulletin, 2023, 39(10): 107-114.

图/表 3

图1 微流控在病原微生物检测中应用

Fig. 1 Application of microfluidic in the detection of pathogenic microorganisms

图2 病毒检测微流控系统 a：用于样本应答病毒核酸检测的全自动离心微流体系统[11]；b：对SARS-CoV-2进行无仪器、基于CRISPR诊断的微流控系统[12]；c：一种利用RT-LAMP检测载体病毒的自供电快速加载微流控芯片[13]

Fig. 2 Microfluidic system for virus detection a: Fully automated centrifugal microfluidic system for sample response viral nucleic acid detection[11]; b: a microfluidic system based on CRISPR diagnosis without instruments for SARS-CoV-2[12]; c: a self-powered fast-loading microfluidic chip using RT-LAMP to detect carrier viruses[13]

图3 细菌检测微流控系统 a：用于血液中大肠杆菌和铜绿假单胞菌的多重检测的微流控平台[18]；b：用于检测单细胞水平耐药性的微流控芯片[20]；c：基于磁分离、酶催化和电化学阻抗分析的鼠伤寒沙门氏菌微流体生物传感器[22]；d：使用LNA/DNA分子信标进行细菌检测和绝对定量的一锅数字微流控系统[23]

Fig. 3 Microfluidic system for bacterial detection a: Microfluidic platform for multiple detection of E. coli and Pseudomonas aeruginosa in blood[18]. b: Microfluidic chip for detecting drug resistance at single cell level[20]. c: Salmonella typhimurium microfluidic biosensor based on magnetic separation, enzyme catalysis and electrochemical impedance analysis[22]. d: One-pot digital microfluidic system using LNA/DNA molecular beacons for bacterial detection and absolute quantification[23]

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微流控技术在病原微生物检测中的研究进展

Research Progress in Microfluidic Technology in the Detection of Pathogenic Microorganisms

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