短小芽孢杆菌对羔羊肠道炎症和屏障功能的影响

doi:10.13560/j.cnki.biotech.bull.1985.2023-0498

摘要/Abstract

摘要：

【目的】本试验旨在研究短小芽孢杆菌对羔羊生长性能及腹泻、血清指标、结肠组织形态、炎性细胞因子和紧密连接蛋白含量的影响。【方法】选取36只初生重3 kg左右的1日龄萨能奶山羊公羔，随机分为4组（每组9个重复，每重复1只羔羊）；每天分别饲喂0（CON组）、1 mL（BP1组）、5 mL（BP5组）、10 mL（BP10组）短小芽孢杆菌菌液，其活菌数为1×10⁸ CFU/mL，试验期14 d。【结果】饲喂短小芽孢杆菌对羔羊末重、平均日增重、平均日采食和料重比均无显著影响（P > 0.05），但均降低了羔羊的粪便评分和腹泻频率（P < 0.05）；BP1组血清中谷草转氨酶含量低于对照组（P < 0.05），二胺氧化酶含量低于对照组和BP5组（P < 0.05），与对照组相比，其它三组均降低了D-乳酸含量（P < 0.05）；BP1组增加了结肠肌层厚度（P < 0.05）；与对照组相比，BP1组、BP5组和BP10组均降低了IL-1β、IL-6、TNF-α含量（P < 0.05），增加了IL-10、TGF-β、IFN-γ、PPAR-γ的含量以及MUC2、Claudin-1、Claudin-4、Occludin和ZO-1的含量（P < 0.05），而且减少了结肠组织中性粒细胞细胞浸润。【结论】在初生羔羊代乳粉中添加短小芽孢杆菌可以降低羔羊腹泻频率，改善结肠组织形态，缓解炎症反应，增强黏膜屏障功能，但对生长性能无显著影响。

关键词: 短小芽孢杆菌, 羔羊, 生长性能, 结肠炎症, 紧密连接蛋白

Abstract:

【Objective】This experiment was conducted to investigate the effects of Bacillus pumilus on growth performance, diarrhea, serum indexes, colonic tissue morphology, inflammatory cytokines and mucosal protein contents of goat kids. 【Method】Thirty-six one-day-old male Saneng dairy goat kids with birth weight of about 3kg were randomly allocated to 4 groups with 9 replicates per group and 1 goat kid per replicate. The goat kids were fed milk replacer containing Bacillus pumilus solution 0（CON group）, 1 mL（BP1 group）, 5 mL（BP5 group）and 10 mL（BP10 group）every day, respectively. The viable count of B. pumilus was 1×10⁸ CFU/mL. The experiment lasted for 14 d. 【Result】Feeding B. pumilus had no significant effect on the final weight, average daily gain, average daily feed intake and feed to gain ratio of goat kids（P > 0.05）, but decreased the fecal score and diarrhea frequency（P < 0.05）. The content of aspartate aminotransferase in the serum of BP1 group was lower than that of control group（P < 0.05）, and the content of diamine oxidase was lower than that of control group and BP5 group（P < 0.05）. Compared with control group, the content of D-lactic acid decreased in the other three groups（P < 0.05）. The thickness of colonic muscle layer increased in BP1 group（P < 0.05）. Compared with the control group, the contents of IL-1β, IL-6 and TNF-α in BP1 group, BP5 group and BP10 group decreased（P < 0.05）. The contents of IL-10, TGF-β, IFN-γ, PPAR-γ, MUC2, Claudin-1, Claudin-4, Occludin and ZO-1 increased（P < 0.05）, and neutrophil cell infiltration in colon tissue decreased. 【Conclusion】Feeding Bacillus pumilus to goat kids may reduce the frequency of diarrhea, relieve inflammatory reaction, and enhance mucosal barrier function of goat kids, but there is no significant effect on growth performance.

Key words: Bacillus pumilus, goat kids, growth performance, inflammation of the colon, tight junction protein

焦帅, 付域泽, 崔凯, 张吉贤, 王杰, 毕研亮, 刁其玉, 张建新, 张乃锋. 短小芽孢杆菌对羔羊肠道炎症和屏障功能的影响[J]. 生物技术通报, 2024, 40(1): 344-352.

JIAO Shuai, FU Yu-ze, CUI Kai, ZHANG Ji-xian, WANG Jie, BI Yan-liang, DIAO Qi-yu, ZHANG Jian-xin, ZHANG Nai-feng. Effects of Bacillus pumilus on the Intestinal Inflammation and Barrier Function of Goat Kids[J]. Biotechnology Bulletin, 2024, 40(1): 344-352.

图/表 9

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营养水平Nutrient level	含量Content
干物质 DM	95.00%
粗蛋白质 CP	22.00%
粗脂肪 EE	24.00%
乳糖 Lactose	35.00%
粗灰分 Ash	9.00%
粗纤维 CF	0.20%
钙 Ga	0.72%
磷 P	0.44%
赖氨酸 Lys	1.70%
氯化钠 NaCl	1.00%
维生素A Vitamin A	25 000 IU/kg

营养水平Nutrient level	含量Content
干物质 DM	95.00%
粗蛋白质 CP	22.00%
粗脂肪 EE	24.00%
乳糖 Lactose	35.00%
粗灰分 Ash	9.00%
粗纤维 CF	0.20%
钙 Ga	0.72%
磷 P	0.44%
赖氨酸 Lys	1.70%
氯化钠 NaCl	1.00%
维生素A Vitamin A	25 000 IU/kg

项目 Item	组别Group				SEM	P值 P-value
项目 Item	CON	BP1	BP5	BP10	SEM	处理Treatment	线性Linear	二次Quadratic
初重 Initial BW/kg	2.83	2.83	3.00	2.88	0.305	0.932	0.726	0.776
末重 Final BW/kg	4.38	4.36	4.66	4.52	0.482	0.918	0.646	0.853
平均日增重ADG/g	111.07	109.56	118.57	116.79	19.192	0.958	0.670	0.992
平均日采食ADFI/g	130.25	124.14	135.74	122.45	12.039	0.679	0.759	0.676
料重比 F/G	1.19	1.17	1.29	1.19	0.173	0.897	0.853	0.753
粪便评分 Fecal score	1.99^a	1.40^b	1.40^b	1.49^b	0.165	0.003	0.008	0.007
腹泻频率 Diarrhea frequency/%	56.25^a	26.79^b	28.72^b	30.50^b	9.378	0.012	0.017	0.026

项目 Item	组别Group				SEM	P值 P-value
项目 Item	CON	BP1	BP5	BP10	SEM	处理Treatment	线性Linear	二次Quadratic
初重 Initial BW/kg	2.83	2.83	3.00	2.88	0.305	0.932	0.726	0.776
末重 Final BW/kg	4.38	4.36	4.66	4.52	0.482	0.918	0.646	0.853
平均日增重ADG/g	111.07	109.56	118.57	116.79	19.192	0.958	0.670	0.992
平均日采食ADFI/g	130.25	124.14	135.74	122.45	12.039	0.679	0.759	0.676
料重比 F/G	1.19	1.17	1.29	1.19	0.173	0.897	0.853	0.753
粪便评分 Fecal score	1.99^a	1.40^b	1.40^b	1.49^b	0.165	0.003	0.008	0.007
腹泻频率 Diarrhea frequency/%	56.25^a	26.79^b	28.72^b	30.50^b	9.378	0.012	0.017	0.026

项目 Item	组别Group				SEM	P值 P-value
项目 Item	CON	BP1	BP5	BP10	SEM	处理Treatment	线性Linear	二次Quadratic
谷丙转氨酶ALT/（U·L^-1）	43.51	34.26	39.07	37.78	3.531	0.104	0.281	0.127
谷草转氨酶AST/（（U·L^-1）	77.53^a	63.56^b	69.68^ab	70.85^ab	4.552	0.047	0.345	0.029
二胺氧化酶DAO/（U·L^-1）	11.66^a	9.43^b	11.23^a	10.63^ab	0.684	0.022	0.556	0.109
D-乳酸 LD/（mmol·L^-1）	8.04^a	6.38^b	6.72^b	6.91^b	0.282	<0.001	0.003	<0.001