纳米材料固定化酶的研究进展

摘要/Abstract

摘要： 纳米材料在蛋白酶及核酶的固定化研究领域进展迅速, 主要包括各种磁性纳米载体及非磁性纳米载体。目前在固定化纳米载体的特性、固定化方法及固定化效果上已进行了广泛探讨。综述以纳米载体的研究现状为基础, 分析纳米载体固定化酶的应用前景及纳米载体固定对酶学性质的影响, 并对该技术的研究进行介绍和展望。

关键词: 纳米材料, 固定化酶, 磁性载体, 非磁性载体, 核酶

Abstract: Immobilization of protease and ribozyme by nanometer carrier are researched as a more useful means, including of the magnetic nanoparticle and nonmagnetic nanoparticles. Currently, the types of immobilized carrier and methods and results of nanoparticles are discussed. In this paper, we describe the current application of immobilized enzyme by nanocarrier, the effect of nanoparticles matrix to enzymatic properties and the prospect of application for the above mentioned technology were introduced, and the direction of the development of nanoparticles immobilization of enzyme was analyzed.

Key words: Nanoparticle cartie, Immobilized enzymes, Magnetic nanoparticles, Non magnetic nanoparticles, Ribozyme

高启禹, 徐光翠, 陈红丽, 周晨妍. 纳米材料固定化酶的研究进展[J]. 生物技术通报, 2013, 0(6): 20-24.

Gao Qiyu, Xu Guangcui, Chen Hongli, Zhou Chenyan. Research Progress of Nanoparticles for Immobilized Enzymes[J]. Biotechnology Bulletin, 2013, 0(6): 20-24.

参考文献

[1] Grubhofer N, Schleith L. Modified ionexchanger resins as specific adsorbent[J]. J Naturwissenschaften, 1953, 40:508-508.
[2] Tosa T, Mori T, Fuse N, et al. Studies on continuous enzyme reactions Part V kinetic and industrial application of aminoacylase column for continuous optical resolution of acyl-dl-amino acids[J]. J Biotechnol Bioeng, 1967, 9:603-615.
[3] Shakeel AA, Qayyum H. Potential applications of enzymes immobili-zed on/in nano materials:A review[J]. J Biotechnology Advan-ces, 2011, 9(5):1016-1028.
[4] 尹艳丽, 王爱玲, 曹健, 等. 纳米载体固定化酶的研究[J]. 现代化工, 2007, 27(9):67-70.
[5] Zuo P, Yu SM, Yang JR, et al. Research progress in support material for immobilization of horseradish peroxidase[J]. J Materials Review, 2007, 21(11):46-49.
[6] 张珊, 游长江, 陶潜, 等. 磁性高分子微球的制备及其应用[J]. 广州化学, 2004, 29(2):45-56.
[7] 刘宇, 郭晨, 王锋, 等. 磁性SiO2纳米粒子的制备及其用于漆酶固定化[J]. 过程工程学报, 2008, 8(3):583-588.
[8] Kouassi GK, Irudayaraj J, McCarty G. Examination of cholesterol oxidase attachment to magnetic nanoparticles[J]. J Nanobiotech-nol, 2005, 3:1-9.
[9] Huang SH, Liao MH, Chen DH. Direct binding and characterization of lipase onto magnetic nanoparticles[J]. J Biotechnol Prog, 2003, 19:1095-1100.
[10] 李咏兰, 吕桂芬, 弓剑, 等. 纳米磁性微粒固定化纤维素酶及水解秸秆的研究[J]. 江西师范大学学报:自然科学版, 2011, 35(6):574-578.
[11] Leslie D, Knecht, Nur Ali, et al. Nanoparticle-mediated remote control of enzymatic activity[J]. J Acs Nano, 2012, 9:1021-1029.
[12] 潘利华, 罗建平, 王贵娟, 等. 磁性纳米氮化铝颗粒固定化β-葡萄糖苷酶的性质[J]. 催化学报, 2008, 29(10):1021-1026.
[13] Ansari SA, Husain Q. Immobilization of Kluyveromyces lactis β-galactosidase on concanavalin A layered aluminum oxide nanoparticles- its future aspects in biosensor applications[J]. J Molecular Catalysis B:Enzyme, 2011, 70:119-126.
[14] Zhou HC, Li W, Shou QH, et al. Immobilization of penicillin g acylase on magnetic nanoparticles modified by ionic liquids[J]. J Chinese Journal of Chemical Engineering, 2012, 20(1):146-151.
[15] Wang JQ, Meng G, Tao K, et al. Immobilization of lipases on alkyl silane modified magnetic nanoparticles:effect of alkyl chain length on enzyme activity[J]. Plos One, 2012, 8(7):1-8.
[16] Talekar S, Ghodake V, Ghotage T, et al. Novel magnetic cross-link-ed enzyme aggregates(magnetic CLEAs)of alpha amylase[J]. Bioresour Technol, 2012, 13(7):542-547.
[17] Zhang LL, Zhao JJ, Jiang JH, et al. Enzyme-regulated unmodified gold nanoparticle aggregation:a label free colorimetric assay for rapid and sensitive detection of adenosine deaminase activity and inhibition[J]. Chem Commun, 2012, 48:10996-10998.
[18] Klyachko NL, Sokolsky-Papkov M, Pothayee N, et al. Changing the enzyme reaction rate in magnetic nanosuspensions by a non-heating magnetic field[J]. Angewandte Chemie, 2012, 51:1-5.
[19] Gardimalla HM, Mandal D, Stevens PD, et al. Superparamagnetic nanoparticle supported enzymatic resolution of racemic carboxyla-tes[J]. J Chem Commun, 2005, 37:4432-4434.
[20] Hong R, Emrick T, Rotello VM. Monolayer-controlled substrate selectivity using noneovalent enzyme-nanoparticle conjugates[J]. J Am Chem Soc, 2004, 126(42):13572-13573.
[21] 刘仁霖, 罗晖, 常雁红. 纳米级酶固定化技术的发展[J]. 科学技术与工程, 2007, 7(7):1411-1415.
[22] Zhang X, Guan RF, Wu DQ, et al. Enzyme immobilization on amin-ofuctionalized mesostructrued cellular foam surfaces, characteriza-tion and catalytic properties[J]. J Molecular Catalysis B:Enzy-matic, 2005, 38:33-43.
[23] 陈金日, 冉旭, 王利. 壳聚糖纳米胶囊固定化α-淀粉酶及其特性的研究[J]. 中国酿造, 2009, 7(208):81-83.
[24] Neri DFM, Balc?o VM, Costa RS, et al. Galactooligosaccharides production during lactose hydrolysis by free Aspergillus oryzae β-galactosidase and immobilized on magnetic polysilox ane-polyvinyl alcohol[J]. J Food Chem, 2009, 115:92-99.
[25] 黄磊, 程振民. 纳米-微米复合泡沫陶瓷固定化脂肪酶[J]. 催化学报, 2008, 29(1):57-62.
[26] Jia H, Zhu G, Wang P. Catalytic behaviors of enzymes attached to nanoparticles:the effect of particle mobility[J]. J Biotechnol Bioeng, 2003, 84:406-414.
[27] Pandey P, Singh SP, Arya SK, et al. Application of thiolated gold nanoparticles for enhancement of glucose oxidase activity[J]. J Langmuir, 2007, 23:3333-3337.
[28] 郭刚军, 马林, 毛学圃, 等. 新型芳香胺-邻醌聚合物的合成及其作为纳米固定化酶载体的研究[J]. 化学学报, 2002, 60(3):499-503.
[29] 王安明, 薛建跃, 万新军, 等. Ag/P(St-MMA)纳米复合高分子微球固定化青霉素酰化酶的研究[J]. 化工科技, 2007, 15(1):9-12.
[30] 赵炳超, 肖宁, 马润宇. 纳米材料MCM-41的制备及其固定化酶的研究[J]. 北京化工大学学报, 2006, 33(1):8-11.
[31] Miletic N, Abetz V, Ebert K, et al. Immobilization of Candida antarctica lipase B on polystyrene nanoparticles[J]. J Macromol Rapid Commun, 2010, 1:71-74.
[32] Chen HL, Yang WZ, Chen H, et al. Surface modification of mitoxantrone-loaded PLGA nanospheres with chitosan[J]. Colloids and Surfaces B:Biointerfaees, 2009, 73:212-218.
[33] 黄赋, 王振刚, 万灵书, 等. 壳聚糖修饰纳米纤维膜表面对氧化还原酶行为的影响[J]. 高等学校化学学报, 2010, 31(5):1060-1064.
[34] Eldin MMS, Zatahry AAE, Al-Sabah MB, et al. Immobilization of β-galactosidase onto copolymers nanoparticles of poly(acrylonitr-ile-co-methylmethacrylate):characterization and application to whey hydrolysis[J]. Nanotechnol Conf USA, 2010.
[35] Liu W, Zhang S, Wang P. Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration[J]. J Biotechnol, 2009, 139:102-107.
[36] Neri DFM, Balcao VM, Carneiro-da-Cunha MG, et al. Immobiliza-tion of β-galactosidase from Kluyveromyces lactis onto a polysilo-xane-polyvinyl alcoholmagnetic(mPOS-PVA)composite for lact-ose hydrolysis[J]. J Catal Commun, 2008, 9:2334-2339.
[37] 蔡宏, 徐颖, 何品刚, 等. 脱氧核糖核酸在电极表面的固定化研究进展[J]. 化学学报, 2004, 32(6):815-820.
[38] 徐桂云, 范金石, 焦奎. 纳米颗粒在DNA固定化中的应用进展[J]. 传感器与微系统, 2008, 27(3):5-8.
[39] 孔德领, 代军, 陈长治, 等. 球形纤维素固定化DNA 制备免疫吸附剂[J]. 高等学校化学学报, 2000, 21(12):1848-1851.
[40] Li J, Hou Ng, Cassell A, et al. Carbon nanotube nanoelectrode array for urltrasensitive DNA detection[J] . Nano Letter, 2003, 3(5):597-602.
[41] 刘盛辉, 陈帆, 奠卫民, 等. 单链膜DNA在氨基乙硫醇单分子金电极上固定化的研究[J]. 高等学校化学学报, 1999, 27(1):38-42.
[42] Cai H, Xu Y, He PG, et al. Indicator free DNA hybridization dete-ction by impedance measurement based on the DNA-doped conduc-ting polymer film formed on the carbon nanotube modified elec-trode[J] . J Electro Analysis, 2003, 15(23-24):1864-1870.