Abstract: The objective of this study is to explore the prevention and recovery measures of strawberry vitrification shoots. Using method of orthogonal design, different combinations of activated carbon, PVA, calcium ion（calcium chloride）were added to subculture medium of the vitrification shoots of ‘Toyonoka’ strawberry to study the effects of strawberry recovery rate. Meanwhile, the physiological and biochemical indexes among recovery plantlets, vitrification shoots and normal plantlets were compared. There were significant differences in recovery rate of vitrification shoots among 9 treatments. The optimum one was No.9（1g/L activated carbon + 2 g/L PVA + 166 mg/L calcium ion）and the recovery rate was 89.07%, followed by the 4^{th treatment（0.5 g/L activated carbon + 166 mg/L calcium ion）, 81.05%, and the 8th treatment（1 g/L activated carbon + 1 g/L polyvinyl alcohol）, 73.87%, respectively. Analysis of variance showed that calcium ion concentration was the most significant impact factor on strawberry vitrification recovery, the second was calcium ion, and polyvinyl alcohol affected least. Significant different physiological and biochemical indexes between the recovery plantlets and vitrification shoots were observed but not between recovery plantlets and normal plantlets. Combined with the recovery rate of vitrification shoots, physiological and biochemical indexes, and the reproduction coefficient, the best recommended recipe for vitrification shoots recovery was the 9th .}