Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (9): 119-123.

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Gene Cloning and Transfer into Tomato of Brucella Outer Membrane Protein OMP31

Wang Jingyan1,2,Wang Jianying1,2,Yu Hua1,Zhao Hongyu1,2,Zhao Liang1,Xin Cuihua1,2,Wang Bin1   

  1. 1. School of Mathematics，Physics and Biological Engineering，Inner Mongolia University of Science and Technology，Baotou 014010；
    2. Institule of Bioengineering Technology，Inner Mongolia University of Science and Technology，Baotou 014010
  • Received:2013-03-26 Revised:2013-09-05 Online:2013-09-05 Published:2013-09-06

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Abstract: Transgenic tomato carrying Brucella suis membrane protein OMP31 gene was constructed for the development of edible vaccine. In the experiment, the Omp31 gene was inserted into a plant expression vector pJG045 using ligation-independent cloning or LIC；the recombinant plasmid was transferred into Agrobacterium tumefaciens AGL-0 by triparental cross；then the transformed Agrobacterium was used to infect tomato cotyledons discs, and transgenic seedlings were identified by antibiotics resistance screening and PCR；5 transgenic tomato plants were obtained.