Cloning and Functional Analysis of MeHDS1 from Cassava Responsing Drought

Abstract

Abstract: The HD-Zip transcription factors are unique to the plant kingdom. They play important roles in plant responsing to the environmental factors. Based on the cDNA of cassava, using RT-PCR technology, one gene sequence was obtained, named it as MeHDS1. The sequence was analyzed, we got a 2 469 bp gene which have integrated ORF, MeHDS1 encoded a protein which contained 822 amino acids（89.07 kD）with an isoelectric point of 5.79. MeHDS1 have typical HD-domain, START domain. It has high homology to GL2 gene of cotton and Arabidopsis, so it might belong to HD-Zip IV subfamily. There have a nuclear localization signal in MeHDS1 protein, and which was localized in the nucleus and cell wall by subcellular localization assay in tobacco epidermal cells. Real-time PCR results showed that, MeHDS1 was upregulated under drought, and its variation of expression was more highly in root cells than that in leaves cells. Through its bioinformatics and expression analysis, we concluded that MeHDS1 may be involved in cassava abiotic stress responsed as a transcription factor.

Key words: HD-Zip transcription factor, Cassava, Expression analysis, Subcellular localization

Yu Xiaoling, Ruan Mengbin, Wang Shuchang, Peng Ming. Cloning and Functional Analysis of MeHDS1 from Cassava Responsing Drought[J]. Biotechnology Bulletin, 2014, 0(10): 76-81.

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