Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (11): 138-141.

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Growth Characteristic and Insect-Resistance of Transgenic Populus alba Populus hopeiensis

Chen Yuchao ,Zhang Li Gong, Lei Gan ,Xiaoyan, Shi Lei ,Song Yuxia   

  1. Key Laboratory of Agricultural Biotechnology of Ningxia, Yinchuan 750002
  • Received:2014-03-07 Online:2014-11-07 Published:2014-11-07

Abstract: The stability of insect-resistance gene Cry3A, phenophase, growth status and insect-resistance of transgenic poplar hybrid(Populus alba×Populus hopeiensis)was investigated, non- transgenic Populus alba×Populus hopeiensis as control. The results showed that the Cry3A gene in transgenic Populus alba×Populus hopeiensis integrated stably. The Cry3A gene could be expressed at RNA level and protein level in root, shoot and leaf. The Bt protein level was significantly different in different tissues, and was highest in shoot. The phenophase and growth status of transgenic Populus alba×Populus hopeiensis changed indistinctively. But it showed distinct insect-resistance.

Key words: Insect-resistance transgenic, Populus alba×, Populus hopeiensis Cry3A gene , Phenophase , Growth status Insect-resistance

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