Application Progress of mazF Gene in Genetic Modification System

Abstract

Abstract: Toxin-antitoxin system（TA）exists in most of the genetic material of bacteria. mazEF is a kind of toxin-antitoxin system in E. coli. MazF encoded by mazF is an mRNA interferase that specifically cleaves free mRNAs at ACA sequences, resulting in inhibited protein synthesis and cell growth arrest. Recently, some scholars have used mazF as a counter-selection marker in genetic modification systems, and achieved modifications of the genome in various strains. In this article, the research advances of E. coli mazF used as counter-selection marker in bacterial genetic modification were reviewed, followed by application of other toxin gene. Finally, perspective of the possible new pathways for developing new genetic modification methods were addressed.

Key words: mazF , Toxin gene , Counter-selection marker , Genetic modification method

Shi Yang,Dong Huina,Zhang Dawei. Application Progress of mazF Gene in Genetic Modification System[J]. Biotechnology Bulletin, 2014, 0(11): 48-54.

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