Abstract: Strain K1-L, isolated from Yuncheng Salt Lake, Shanxi Province, could swim on the 2%-10% MGM medium. The strain K1-L was Gram-negative with polar flagellum and was 0.4 μm ×(1-2)μm under the scanning electron microscope. Strain K1-L appeared grey, rounded colony. Its optimum salt concentration, temperature and pH ranges were 2%-6%, 30-40℃ and 6.0-8.0. The GC content is 47.06%. After comparing the traditional characteristics of morphology, physiological biochemistry and 16S rRNA sequence analysis, we discovered that the strain K1-L was highly homologous to the Vibrio diabolicus HE800^T and Vibrio rotiferianus LMG 21460^T. The maximum similarity was 99%. It was asserted that K1-L was belonged to the genus Vibrio, we proposed the name Vibrio sp. K1-L for this strain.

Key words: Yuncheng salt lake, Vibrio, Halotolerant, Phylogenetic analysis