Transcriptional Activation Identification of the Transcription Factor HcSCL13 from Halostachys caspica and the Construction of Plant Expression Vector

Abstract

Abstract: HcSCL13 gene of the GRAS family from Halostachys caspica(GenBank accession number：KC68640)was amplified and ligated to the pGBKT7 vector by homologous recombination to construct the bait vector pGBKT7-HcSCL13, and then transformed into the competent yeast Y2HGold. Self-activation of recombinant bait vector in yeast two-hybrid system was measured. The results showed that the bait protein had not toxic to Y2HGold and could activate all the reporter genes. Therefore, we proposed HcSCL13 was a transcription factor with a transcriptional activation domain. The plant over-expression vector and subcellular localization expression vector were successfully constructed for further studying its biological function.

Key words: Halostachys, Caspica Transcription factor HcSCL13, Transcriptional activation, Plant expression vector

Zhou Lianjie, Zhang Fuchun, Wang Yan. Transcriptional Activation Identification of the Transcription Factor HcSCL13 from Halostachys caspica and the Construction of Plant Expression Vector[J]. Biotechnology Bulletin, 2014, 0(5): 76-82.

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