Abstract: In order to provide more gene resources for biological control, we used the cry1 class full-length primers and genomic DNA of 300 Bacillus thuringiensis strains which stored in our laboratory as the templates to identify cry1 gene-types by PCR. The strain V4 was identified containing a cry1Ea gene by PCR-RFLP, further study found that the strain also contained cry2Aa gene. The genes from the strain V4 were cloned, respectively. The two genes were designated as cry1Ea12 and cry2Aa16 by International Nomenclature Committee of Bacillus thuringiensis. Furthermore, the two genes were respectively transformed into Escherichia coli Rosetta（DE3）strain and the toxicity of the two genes was evaluated. The results showed that the insecticidal activity of the proteins is not very efficient, but the weights of the test insects are inhibited obviously, and the insecticidal activity of the protein of the strain V4 is much higher.

Key words: Bacillus thuringiensis, Insecticidal crystal protein cry1Ea12 gene cry2Aa16 gene , Insecticidal activity