Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (9): 131-135.

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Variance Analysis of Different Mating Type Strains in Mononuclear Protoplast of Hypsizygus marmoreus

Pan Yue1,2,Chen Hui2,Feng Zhiyong1,2,Zhao Jing3   

  1. 1. College of Life Sciences，Nanjing Agricultural University，Nanjing 210095;
    2. National Research Center for Edible Fungi Biotechnology and Engineering，Shanghai Key Laboratory of Agricultural Genetics and Breeding， Institute of Edible Fungi，Shanghai Academy of Agricultural Sciences，Shanghai 201403;
    3. Shanghai Bright Esunyes Bio-tech Co. Lyd，Shanghai 201408
  • Received:2014-02-12 Online:2014-09-15 Published:2014-09-07

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Abstract: Tow Hypsizygus marmoreus strains were studied by protoplast isolation and mating-type identification. Metabolic characteristics of different mating type strains were studied. Results showed that each of the 2 Hypsizygus marmoreus strains had 2 mating type. The ratio of two mating-type monokaryons of 2 strains was not 1∶1 and the proportion of mating types had different phenomenon. Different kinds of mating type had different hyphae form and there was difference between the conductivity, the pH, the content of reducing sugar of the broth and the mycelial biomass in different mating type strains, which indicated that hyphae form and metabolic characteristic had relations with mating factors in this two strains.

Key words: Hypsizygus marmoreus , Monokaryon protoplast, Mating type Hyphae form, Metabolic characteristic