The Production of Phenolic Compounds of Inonotus obliquus by Fungal Elicitor Introducing and Its Study of Biochemical Mechanism

Abstract

Abstract: In order to study the effects of fungal elicitor on secondary metabolites of Inonotus obliquus, using shaking flask method, the fungal elicitor was added to the liquid cultures of Inonotus obliquus, detected the accumulation of secondary metabolites, and the effects of fungal elicitor on Inonotus obliquus nitric oxide synthase（NOS）activity, discussed the influence of elicitors to Inonotus obliquus liquid fermentation process and the accumulation of secondary metabolites. The results showed that the addition of fungal elicitor could improve the metabolic rate of Inonotus obliquus and the accumulation of polyphenols. The intracellular and extracellular polyphenols were up to 102.15 mg/L and 311.91 mg/L, and could also significantly improve the activity of NOS, NOS activity was up to 151.93 U/mg prot, nitric oxide content in the fermentation liquid was up to 256.28 μmol/L. This showed, fungal elicitor could effectively active the biosynthesis pathway of secondary metabolites of Inonotus obliquus.

Wei Zhiwen,Sun Yong, Wang Fei. The Production of Phenolic Compounds of Inonotus obliquus by Fungal Elicitor Introducing and Its Study of Biochemical Mechanism[J]. Biotechnology Bulletin, 2014, 0(9): 136-141.

References

［1］Mizuno T, Zhuang C, Abe K, et al. Antitumor and Hypoglycemic Activities of Polysaccharides from the Sclerotia and Mycelia of Inonotus obliquus（Pers.：Fr.）Pil.（Aphyllophoromycetideae）［J］. International Journal of Medicinal Mushroom, 1999（1）：301-316.
［2］Lee IK, Yun BS. Hispidin analogs form the mushroom Inonotus xeranticus and their free radical scavenging activity［J］. Bioorganic & Medicinal Chemistry Letters, 2006, 16（9）：2376-2379.
［3］Lee IK, Yun BS. Highly oxygenated and unsaturated metabolites providing a diversity of hispidin class antioxidants in the medicinal mushrooms Inonotus and Phellinus［J］. Bioorganic & Medicinal Chemistry, 2007, 15（10）：3309-3314.
［4］Lee IK, Seok SJ, Kim WK, et al. Hispidin derivatives from the mushroom Inonotus xeranticus and their antioxidant activity［J］. Journal of Natural Products, 2006, 69（2）：299-301.
［5］Kukulianskaia TA, Kurchenko NV, Kurchenko VP, et al. Physicochemical properties of melanins produced by Inonotus obliquus（“chagi”）in the nature and the cultivated fungus［J］. Prikladanya Biokhimiya i Mikrobiologya, 2002, 38（1）：68-72.
［6］Yusoo S, Yutaka T, Terazawa M. Triterpenoids, steroids, and a new sesquiterpene from Inonotus obliquus（Pers.：Fr.）Pilat［J］. Internatioanl Journal of Medicinal Mushroom, 2002, 4（2）：77-84.
［7］Jung JY, Lee IK, Seok SJ, et al. Antioxidant polyphenols from the mycelial culture of the medicinal fungi Inonotus xeranticus and Phellinus linteus［J］. Journal of Applied Microbiology, 2008, 104（6）：1824-1832.
［8］Zhang DL, Hamauzu Y. Phenolic compounds and their antioxidant properties in different tissues of carrots（Daucus carota L.）［J］. Food, Agriculture and Environment, 2004, 2（1）：95-100.
［9］Zhao J, Davis LC, Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites［J］. Biotechnol Adv, 2005, 23（4）：283-333.
［10］Shwab EK, Keller NP. Regulation of secondary metabolite production in filamentous ascomycetes［J］. Mycological Research, 2008（112）：225-230.
［11］徐茂军, 董菊芳, 朱睦元. NO通过水杨酸（SA）或者茉莉酸（JA）信号途径介导真菌诱导子对粉葛悬浮细胞中葛根素生物合成的促进作用［J］.中国科学（C辑：生命科学）, 2006（1）：66-75.
［12］Gorinstein S, Zemser M, Haruenkit R, et al. Comparative content of total polyphenols and dietary fiber in tropical fruits and persimmon［J］. Journal of Nutritional Biochemistry, 1999, 10（6）：367-371.
［13］Lotito SB, Frei B. Relevance of apple polyphenols as antioxidants in human plasma：Contrasting in vitro and in vivo effects［J］. Free Radical Biology & Medicine, 2004, 36（2）：201-211.
［14］曹炜, 索志荣. Folin-Ciocalteu比色法测定蜂蜜中总酚酸的含量［J］.食品与发酵工业, 2003, 29（12）：80-82.
［15］刘倩, 何法林, 江春花, 等.松茸多糖两种测定方法的比较研究［J］.时珍国医国药, 2010, 21（5）：1058-1059.
［16］刘晓庚, 曾尚义, 陈学恒, 等.光度滴定法同时测定酱油中游离酸和游离氨基氮的研究［J］.食品科学, 1996, 17（3）：50-55.
［17］田志梅, 张永顺.紫外分光光度法快速测定液体奶、奶粉中蛋白质含量［J］.中国卫生检验杂志, 2008, 18（2）：263-264.
［18］Modolo LV, Cunha FQ, Braga MR, et al. Nitric oxide synthase-mediated phytoalexin accumulation in soybean cotyledons in response to the Diaporthe phaseolorum f. sp. meridionalis elicitor［J］. Plant Physiol, 2002, 130（3）：1288-1297.
［19］Xu MJ, Dong JF, Zhu MY. Nitric oxide mediates the fungal elicitor-induced hypericin production of Hypericum perforatum cell suspension cultures through a jasmonic-acid-dependent signal pathway［J］. Plant Physiol, 2005, 139（2）：991-998.