Preliminary Investigation on Antimicrobial of the Epidermal Mucus Secretion of Roughskin Sculpin（Trachidermus fasciatus）

Abstract

Abstract: The epidermal mucus samples from roughskin sculpin（Trachidermus fasciatus）were extracted with aqueous, acidic and organic solvents to identify potential antimicrobial acitivity by the methods of Kirby-Bauer and two fold dilution. The crude acidic extracts only showed antibacterial activity against all six tested pathogens and the antibacterial ability of descending order is Aeromonas veronii, Staph epidermidis, Staph soparophytics, Aeromonas hydrophila, Citrobacter freundii, Staph warneri. The protein profiles of the acidic extracts mucus on tricine SDS-PAGE gel showed proteins was less than 95 kD, and the highest levels was between 34 kD to 55 kD. Preliminary studies indicated that the epidermal mucus samples from roughskin sculpin（Trachidermus fasciatus）extracted by acidic played an major role to defense against these pathogens, and to determine the antimicrobial protein remains to be further analyzed.

Key words: Epidermal mucus , Roughskin sculpin , Antimicrobial acitivity , Antimicrobial proteins

Qiu Jin,Pan Liande,Zhang Shuai. Preliminary Investigation on Antimicrobial of the Epidermal Mucus Secretion of Roughskin Sculpin（Trachidermus fasciatus）[J]. Biotechnology Bulletin, 2014, 0(9): 195-200.

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