Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 87-92.

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Extraction of High Quality Total RNA from Ammopiptanthus mongolicus with Improved Trizol Method

Chen Jing, Gao Fei, Zhou Yijun, Zhang Zichen, Li Zhanglei, Cao Yuzhen, Zhang Zhiwei   

  1. （College of Life and Environmental Sciences，Minzu University of China，Beijing 100081）
  • Received:2013-04-24 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

Total RNA was extracted using an improved Trizol method, with the leaves and roots of xylophyta, Ammopiptanthus mongolicus （Maxim）Cheng f. The result showed that compared to traditional Trizol method, the improved Trizol method can effectively remove residual guanidinium isothiocyanate, beta-mercaptoethanol in extraction process, as well as the sugar and polyphenols of the plant itself, and high-quality total RNA was obtained. The improved Trizol method has been successfully applied to transcriptome database constrction of A. mongolicus. It also provided a reference for xylophyta to extract high-quality RNA.

Key words: Total RNA extraction, A. mongolicus, Improved Trizol method