The Overexpression of PELO in Human Embryonic Stem Cells

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 170-176.

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The Overexpression of PELO in Human Embryonic Stem Cells

Hu Xulin, Kee Kehkooi

（Center for Stem Cell Biology and Regenerative Medicine，School of Medicine，Tsinghua University，Beijing 100084）

Received:2013-05-10 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

Abstract

Abstract:

Using full length human PELO cDNA as template, TOPO cloned PELO gene to pENTR-D-TOPO vector to form the pENTR-D-PELO vector, then performed the MultiSite Gateway LR recombination reaction between pENTR-5’-EF1α and pENTR-D-PELO to get the EF1α-PELO-p2k7 expression vector. PELO overexpression lentivirus was produced in 293FT cells and ransducing human embryonic stem cells to overexpress PELO.

Key words: PELO, Overexpression, Embryonic stem cells, Lentivirus, Homologus recombinant

Hu Xulin Kee Kehkooi . The Overexpression of PELO in Human Embryonic Stem Cells[J]. Biotechnology Bulletin, 2013, 0(10): 170-176.

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The Overexpression of PELO in Human Embryonic Stem Cells

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