Optimization of Agrobacterium-mediated Transformation of Cryptococcus neoformans vps41 Δ and Construction of a Insertional mutagenesis Library

Abstract

Abstract: Cryptococcus neoformans is a major human opportunistic fungal pathogens. The previous work has shown that VPS41 gene contributes to virulence by mediating nitrogen starvation response. In this study, major factors affecting the efficiency of Agrobacterium tumefaciens-mediated transformation of vps41Δ were systematically investigated, and optimized parameters were used to construct an insertional metagenesis library of the deletion mutant containing over 5 000 transformants. The availability of this insertional library could facilite the screening and identification of other genes involved in the signaling pathway of starvation response of C. neoformans.

Key words: Cryptococcus neoformans, Agrobacterium tumefaciens, vps41Δ, T-DNA, Mutant library

Song Yingjin, Liu Xiaoguang. Optimization of Agrobacterium-mediated Transformation of Cryptococcus neoformans vps41 Δ and Construction of a Insertional mutagenesis Library[J]. Biotechnology Bulletin, 2013, 0(12): 151-155.

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