Research on the Method of Extraction of Lycopeneprepared by Optimizated Ethanol Using Response Surface Analysis

Abstract

Abstract: To research the methond of extraction of lycopene repared by optimizated ethanol using response analysis. The effects of ethanol pretreatment, extracting solvent, microwave assisted extraction and ultrasonic assisted extraction, were evaluated by using a central composite rotatable design. Using the dehydration processing tomato sauce can obviously improve the extraction efficiency of Lycopene. Then,the central composite design and response surface analysis were used to determine the optimal levels of the main factors. The optimal extracting conditions and lycopene yields of ultrasound assisted extraction technology were described as follows: the extraction time, 490 s ；the ratio of solvent to tomato sauce, 10.1: 1(V/W)；the ratio of anhydrous ethanol to tomato sauce, 2.05: 1(V/W)； extraction power, 405 W ； lycopene yields, 94.42%. The optimal extracting conditions and lycopene yields of microwave assisted extraction technology were described as follows: the extraction time, 372.6 s ；the ratio of solvent to tomato sauce, 10.1: 1(V/W)；the ratio of ethanol to tomato sauce, 2.11: 1(V/W)； extraction power, 569.5 W ；lycopene yields, 81.51%. Results showed that pretreated by ethanol, power, extracting time and extracting solvent were the main affecting factors.

Key words: Tomato sauce, Lycopene, Microwave treatment, Ultrasonic treatment

Shen Haitao, Wang Aiying, Zhu Jianbo. Research on the Method of Extraction of Lycopeneprepared by Optimizated Ethanol Using Response Surface Analysis[J]. Biotechnology Bulletin, 2014, 0(1): 83-92.

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