Identification of Cashmere and Sheep down by Microarray Technology

Abstract

Abstract: This study is to explore the method for identification of cashmere and sheep down by gene chip, thereby enabling rapid, highthroughput identification of cashmere and sheep down. According to the specificity of the genetic material of cashmere and sheep down, cyt b was selected as the target gene, a pair of probes that can identify cashmere and sheep down was designed in the universal primers interval of cyt b gene. Then PCR products of different proportions derived from sheep were hybridized with probe. Results showed that in goat derived PCR products, when content of PCR products derived from sheep was 3%, hybridization signal was still visible. It indicates that the gene chip method can be used to qualitatively identify the cashmere and sheep down, and has a very high specificity.

Key words: Cashmere, Sheep down, Mitochondria DNA, Cyt b gene, Gene chip

Lü Xuefeng, Abliz Ablimit Pa Naer, Tao Weidong, Xing Weiting, Cai Fula, Zheng Wenxin. Identification of Cashmere and Sheep down by Microarray Technology[J]. Biotechnology Bulletin, 2014, 0(1): 196-200.

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