Abstract: A cDNA sequence of Musca domestic ubiquitin-conjugated enzyme（MD-E2）was obtained by in EST technology. Some characters of the MD-E2 gene and encoded protein sequence were predicted and analyzed by bioinformatics methods in the following aspects, such as general physical and chemical properties, hydrophobicity, signal peptide, secondary structure and localization sites in cells. Results showed that the MD-E2 contains a complete ORF（480bp）encoding 159 amino acid with a predicted molecular weight about 18.117 9 kD and pI 8.64. The protein encoded by MD-E2 gene has no signal peptide, and no transmembrane domains, and it is a hydrophilic protein which is mainly located in cell nucleus. The secondary structures are mainly composed of random coil.