Progress of Pichia pastorisEngineering Bacteria on High-Density Fermentation

Abstract

Abstract: High-density fermentation has been a key technique tache to improve the expression level of the protein, and fermentation process is an important factor in high-density fermentation. The Pichia pastoris protein expression on its genetic characteristics, host strains, expression vectors and the expression of heterologous proteins in the areas of basic researches were reviewed. In addition, we briefly discussed high-density fermentation of Pichia pastoris in the selection of engineering bacteria, designation and optimization of culture medium, control of the key biochemical parameters of fermentation process, and methanol induction. In the end, the problems existing in industry high-density fermentation progress were put forward, and the development trend was prospected.

Min Zhaosheng, Guo Huiming, Yan Xu, Hong Housheng. Progress of Pichia pastorisEngineering Bacteria on High-Density Fermentation[J]. Biotechnology Bulletin, 2014, 0(3): 42-49.

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