The Cloning and Expression Analysis of PwARP-1 in Picea wilsonii

Abstract

Abstract: An auxin repressed protein gene PwARP-1 is cloned from Picea wilsonii. The full length cDNA of PwARP-1 is obtained by RACE-PCR assays based on the cDNA library of Picea wilsonii and EST fragment of PwARP-1. Bioinformatic tools are applied for the prediction of PwARP-1. PwARP-1 encodes a protein with 155 amino acids. The molecular weight is 17.1 kD and theoretical pI is 10.07 and the protein is rich in random coil. The tissue-specific expressions and the response to phytohormones of PwARP-1 were investigated by RT-qPCR assays. The tissue-specific expression assays suggested that the expression level in pollen was the highest among all tissues and the expression level in seed was the lowest. During seed germination, the expression of PwARP-1 was increased, peaked at the 10th day and then decreased. Furthermore, the expression of PwARP-1 was significantly inhibited by Auxin and Gibberellin. However, the expression level of PwARP-1 is up-regulated by Brassinolide and drought stress treatment. The expression level of PwARP-1 declined and then up-regulated under salt stress, methyl jasmonate, and abscisic acid treatments. These results indicate that PwARP-1 has a potential function in seed germination and responding to stresses and phytohormone treatments.

Key words: Picea wilsonii, Auxin-repressed protein, Expression analysis, Phytohormone

Sun Fan, Luo Chaobing, Zhou Yanni, Zhang Lingyu. The Cloning and Expression Analysis of PwARP-1 in Picea wilsonii[J]. Biotechnology Bulletin, 2014, 0(4): 64-70.

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