Exploiting Cell-free System of Recombinant E. coli to Synthesize Pyrroloquinoline Quinone

Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (4): 164-168.

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Exploiting Cell-free System of Recombinant E. coli to Synthesize Pyrroloquinoline Quinone

Sun Jiguo¹, Han Zengye¹, Ge Xizhen², Tian Pingfang¹,

(1. College of Life Science and Technology，Beijing University of Chemical Technology，Beijing 100029；
2. College of Biochemical Engineering，Beijing Union University，Beijing 100023)

Received:2013-11-25 Online:2014-04-29 Published:2014-04-29

Abstract

Abstract: In this study, cell-free system was developed to overproduce pyrroloquinoline quinone(PQQ). The PQQ gene cluster from Klebsiella pneumoniae was subcloned into vector harboring lac promoter and a recombinant Escherichia coli was acquired. Subsequently cell homogenate was prepared which showed ~30% increase of PQQ yield compared with in vivo biosynthesis. Not only revealing the presence of intracellular velocity-limiting reaction(s)that retards PQQ biosynthesis, this study also suggests that cell-free system can address this issue. Hence, this study provides basis for further enhancement of PQQ production.

Key words: E. coli, Cell-free system, Pyrroloquinoline quinine, Velocity-limiting reaction

Sun Jiguo, Han Zengye, Ge Xizhen, Tian Pingfang,. Exploiting Cell-free System of Recombinant E. coli to Synthesize Pyrroloquinoline Quinone[J]. Biotechnology Bulletin, 2014, 0(4): 164-168.

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Exploiting Cell-free System of Recombinant E. coli to Synthesize Pyrroloquinoline Quinone

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