Colonization of Bacillus subtilis Y13 in Camellia oleifera Leaves and Its Effect on Native Bacteria

Abstract

Abstract: The colonization capacity of strain Y13 in the Camellia oleifera, was investigated by marking with rifampicin, periodical sampling, and recovering bacteria through diluting-plate method, as well as the effects of Y13 on native bacterial population were also studied by diluting-plate and 16S rDNA methods, which would provide a basis for ecological control of camellia anthracnose. The results showed strain Y13^Rdisplayed good colonization in camellia oleifera, 30 days after inoculation, 6.60×10³CFU/g and 3.56×10³CFU/g strains Y13^Rcould be detected in healthy and diseased leaves, respectively. Endophytic bacteria isolated from Camellia oleifera belong to 5 genera of 7 species, Bacillus, Lysinibacillus, Klebsiella, Sphingomonas and Pseudomonas, Bacillus and Lysinibacillus was the most prevalent genera. The group and amount of predominant species had evident distinction in healthy and diseased leaves. The diversities of bacteria in the Camellia oleifera leveas were improved by Y13 treatment and suppressed the pathogen propagation.

Key words: Camellia anthracnose, Endophytic antagonistic bacteria, Colonization, Native bacteria

Wang Ruiqin, Zhou Guoying, Liu Junang, Li Dongqin, Meng Qingmin. Colonization of Bacillus subtilis Y13 in Camellia oleifera Leaves and Its Effect on Native Bacteria[J]. Biotechnology Bulletin, 2014, 0(6): 162-167.

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