Abstract: Food poisoning incidents have occurred by the pollution of food-borne pathogenic bacteria frequently. PCR meet the requirements of the rapid detection of pathogenic bacteria. The three primers was designed by primer 5.0 software of Enterohemorrhagic Escherichia coli O157:H7 rfbE gene, Staphyloccocus aureus nuc gene, Salmonella hilA gene. Three amplified segments of rfbE gene, nuc gene, hilA gene were 287 bp, 354 bp, 468 bp. The specificity and sensitivity of primers were verified by single, duplex and triplex-PCR reaction, and by detecting the foods artificially infected with the three pathogens. The results showed that three food-borne bacterial pathogens were amplified purpose fragments, and no non-specific fragments were amplified. It was obvious that there were the special fragments of the artificial food infected with three pathogens by PCR test. The three primers could be used for PCR detection of target genes about three bacteria.

Key words: Food-borne pathogenic bacteria, Enterohemorrhagic, Escherichia coli O157:H7 , Staphyloccocus aureus Salmonella PCR