Study on Antimicrobial Resistance and Plasmid-mediated Quinolone Resistance of Foodborne Salmonella Isolates

Abstract

Abstract:

A total of 638 food samples were collected randomly to determine the prevalence of Salmonella. The overall percentage of Salmonella prevalence was 9.7%（n=62）. Among 16 different serotypes identified, S. Anatum was most common. The isolates were frequently resistant to sulfamethoxazole, trimethoprim/sulfamethoxazole, streptomycin, and ciprofloxacin. Five different types of amino acid substitutions were identified in quinolone resistance-determining region（QRDR）of GyrA and ParC of 16 ciprofloxacin-resistant isolates. Double mutations of Ser83Phe and Asp87Gly in GyrA accompanied by an additional mutation of Ser80Arg in ParC were found most frequently. The qnr genes were present in seven（11.3%）of 62 isolates, and among them, two isolates carried qnrA and five carried qnrS. qnrB, qnrC, and qnrD were not detected in the isolates in this study. Eight isolates were positive for aac（6'）-Ib, of which three isolates carried the-cr variant. The results suggested the diversity of serotype distribution, serious situation of antimicrobial resistance and the presence of plasmid-mediated quinolone resistance（PMQR）genes in foodborne Salmonella from Xinxiang.

Key words: Foodborne, Salmonella, Antimicrobial resistance, Plasmid-mediated, Quinolone

Liu Guishen, Yu Tao. Study on Antimicrobial Resistance and Plasmid-mediated Quinolone Resistance of Foodborne Salmonella Isolates[J]. Biotechnology Bulletin, 2014, 0(8): 202-207.

References

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