Effects of Crotonaldehyde on the Protein Expression of Rice Suspension Cells

Abstract

Abstract: In order to study the important proteins related to cell cycle of rice, the effect of crotonaldehyde on the protein expression of suspension cell line of rice was investigated. The proteins were separated by the method of two dimensional electrophoresis, the different protein spots were analyzed by PDQuest software, and then the different protein spots were identified by LCQ-Fleet ion trap and database searching. The results showed that there were forty-five protein spots expressed differently, and twenty-six of them were identified by mass spectrometry and database analysis. The predicted function of these proteins was related to stress response, protein synthesis and processing, signal transduction, metabolism membrane function. Some of the proteins, including small molecular proteins, Skp1, 26S proteasome, cell division cycle protein 48, glucosamine-6-phosphate acetyltransferase and sucrose synthase, were related with the cell cycle process. Other identified proteins should be study further to explore its function in cell cycle of rice. The normal cell cycle activity was effected by crotonaldehyde, some of the identified proteins were related with regulation of cell cycle. Other proteins related to cell differentiation had no reports about their function to cell cycle;these proteins should be study further to explore the mechanism of cell cycle regulation.

Key words: Crotonaldehyde, Rice suspension cells, Different proteomics, Cell cycle

Liu Yueping, Guo Bei, Hu Hao, Zhang Guoqing. Effects of Crotonaldehyde on the Protein Expression of Rice Suspension Cells[J]. Biotechnology Bulletin, 2014, 0(10): 119-127.

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