Optimization of Agrobacterium-mediated Transformation in Castor(Ricinus communis L.)

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.022

Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (5): 140-145.doi: 10.13560/j.cnki.biotech.bull.1985.2015.05.022

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Optimization of Agrobacterium-mediated Transformation in Castor(Ricinus communis L.)

Li Wei, Zhai Yujia, Li Pengcheng, Wang Changlu

（Key Laboratory of Food Nutrition and Safety，Tianjin University of Science and Technology，Tianjin 300457）

Received:2014-09-23 Online:2015-05-18 Published:2015-05-18

Abstract

Abstract: The main factors affecting the efficiency of Agrobacterium infection were optimized, and the optimal infection conditions were 5-day-old pre-cultured explants cultured in bacteria solution with OD₆₀₀ 0. 8. Infected explants after 5 d of co-culture were transferred to medium with 250 mg/L Kan for bud induction and many strains with Kan resistance ability were obtained. By adding 20 mg/L AS, 124mg/L Na₂S₂O₃ and 152. 4 mg/L DTT in the common culture medium, browning phenomenon was effectively inhibited and survival rate of resistant shoots was enhanced. Regenerated plants with resistance to Kan were verified by PCR and Southern blot test. Total 18 positive transformants were obtained.

Key words: Castor（Ricinus comunis L.）, Agrobacterium infection, transformation system

Li Wei, Zhai Yujia, Li Pengcheng, Wang Changlu. Optimization of Agrobacterium-mediated Transformation in Castor(Ricinus communis L.)[J]. Biotechnology Bulletin, 2015, 31(5): 140-145.

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Optimization of Agrobacterium-mediated Transformation in Castor(Ricinus communis L.)

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