Rapid Detection and Identification of Fusarium oxysporum f. sp. niveum

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.009

Abstract

Abstract: Based on the genome comparison analysis between Fusarium oxysporum f. sp. niveum(Fon)and other closely-related pathogens, the specific sequences of Fon were obtained. Then we developed specific primers that could amplify Fon-specific DNA bands. Combined the Fon-specific primers with the universal primer of Fusarium oxysporum (W106R/W106S), we established a duplex PCR method that might rapidly and accurately detect Fon by 1 PCR reaction, which is beneficial to technically support the rapid identification of Fon by method of molecular detection..

Key words: Fusarium oxysporum f. sp. niveum, duplex PCR, Fusarium oxysporum

Cao Yuexia, Ling Jian, Xie Bingyan, Yang Yuhong. Rapid Detection and Identification of Fusarium oxysporum f. sp. niveum[J]. Biotechnology Bulletin, 2015, 31(7): 58-63.

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