Prediction and Analysis of Genes Encoding c-di-GMP-Metabolizing Enzymes in Xanthomonas oryzae pv. oryzae

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.016

Abstract

Abstract: Cyclic di-GMP, a ubiquitous bacterial second messenger that regulates diverse cellular processes including virulence, is synthesized by diguanylate cyclase(DGC)with the GGDEF domain and degraded by phosphodiesterase(PDE)with the EAL or HD-GYP domain, respectively. To identify genes encoding DGCs and PDEs in Xanthomonas oryzae pv. oryzae, prediction and analysis of the GGDEF, EAL and HD-GYP domain proteins in three genome-sequenced strains PXO99^A, MAFF311018 and KACC10331 was comparatively performed in this study. Twenty-five to twenty-six GGDEF, EAL and HD-GYP domain protein genes were identified from each of three strains. Eighteen motif-conserved proteins were predicted to be the active DGCs or PDEs to metabolize c-di-GMP, whereas the remaining with the degenerate motifs might be the c-di-GMP receptors. Moreover, the majority of proteins were demonstrated to contain the input sensor domains to perceive the environmental stimulation and thereby modulate the activity of downstream output domains. The part of proteins was experimentally validated for their functions in the c-di-GMP signaling and regulation of virulence in X. oryzae pv. oryzae.

Key words: Xanthomonas oryzae pv. oryzae, c-di-GMP signaling, diguanylate cyclase, phosphodiesterase

Xue Dingrong, Tian Fang, Li Haiyun, Yang Fenghuan, Chen Huamin, He Chenyang. Prediction and Analysis of Genes Encoding c-di-GMP-Metabolizing Enzymes in Xanthomonas oryzae pv. oryzae[J]. Biotechnology Bulletin, 2015, 31(11): 131-138.

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