Abstract: Ligustrum lucidum'Yi Feng', a wild and variant species, because its resource was very scarce, and routine methods could not achieve large-scale propagation during a short period of time, a relatively perfect tissue culture and rapid propagation system for massive production was established in this research with axillary buds of young branch cuttings as explant materials. The results showed that, the optimal initial medium was WPM+0.3 mg/L KT +0.1 mg/L 6-BA +0.05 mg/L NAA, and the germination frequency of axillary buds reached 95.6%；the optimal proliferation medium was WPM+0.5 mg/L KT +1.0 mg/L 6-BA +0.05 mg/L NAA, and its average proliferation coefficient reached 5.63；the optimal rooting medium of shoots was 1/2WPM+1.5 mg/L IBA +0.1 mg/L NAA. After about 20 days, the overall frequency of shoots rooting reached about 95.4%. And then, more than 95% of the plantlets could survive on the substrate soil in the end. The system can satisfy the need of industrial production, and the plantlets can steadily inherit the outstanding variation character of parents.

Key words: Ligustrum lucidum 'Yi Feng', rapid propagation, proliferation culture