A Research of L-theanine Synthesis with Two Feeding Methods of L-glutamine Using γ-glutamyltranspeptidase

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.022

Abstract

Abstract: The optimization of L-theanine production by γ-GGT(gamma-glutamyltranspeptidase)was investigated.The constant speed feeding strategy was performed using 200 mmol/L L-glutamine and 2 mol/L ethylamine hydrochloride as the initial substrates，under the condition of 37℃，pH10.0，100 mmol/L L-glutamine was added every 2 hours and the reaction sustained for 14 hours.Eventually the total concentration of L-glutamine and L-theanine were 900 mmol/L and 573.2 mmol/L respectively.The conversion rate of L-theanine reached 63.7%，and the production intensity was 40.9 mmol/(h·L).The variable speed feeding process conducted for 15 hours with the same initial conditions，and the adding L-glutamine to the initial concentration of 200 mmol/L in every 2 hours.After 15 hours，600 mmol/L of L-glutamine was converted to L-theanine with a final concentration of 445.8 mmol/L and the conversion rate and production intensity reached 74.3% and 29.7 mmol/(h·L).

Key words: γ-glutamyltranspeptidase, L-theanine, constant speed feeding, constant speed feeding

FU Jia-yi, CHEN Sheng, WU Jing. A Research of L-theanine Synthesis with Two Feeding Methods of L-glutamine Using γ-glutamyltranspeptidase[J]. Biotechnology Bulletin, 2016, 32(2): 165-171.

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