Abstract: A rapid propagation system was established by tissue culture in order to provide raw materials for better use and development of Dendrobium officinale. Using stem segments of D. officinale as explants, effects of the differential kinds and concentrations of plant growth regulators and natural additives on the induction of adventitious shoots growth and proliferation of cluster shoots were studied；and the optimal mediums at different culture stages were selected. Results showed that the optimal hormone ratio for induction of adventitious shoots was NAA 1.0 mg/L and BA 2.0 mg/L, a combination of NAA 0.5 mg/L, BA 2.0 mg/L, banana juice 100 g/L and activated carbon 100 g/L was the optimal medium for the proliferation of cluster shoots. It is feasible to obtain the efficient and stable plantlets by establishing high efficiency and rapid propagation system from stem segments of D. officinale.

Key words: Dendrobium officinale, adventitious shoot, cluster shoot, rapid propagation