The Mechanism of Lentinan Enhancing the Resistance of Cucumber Seedlings to Colletotrichum orbiculare

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.015

Abstract

Abstract: This study is to investigate the inducing effect of lentinan on the defense responses of cucumber(Cucumis sativus L. cv. Jinfeng)seedling leaves. The seedlings were treated with 0.75 g/L of lentinan. The concentration of H₂O₂ and salicylic acid in cells of leaves increased rapidly and reached the peak levels at 10 min and 4 h, respectively. Afterwards, the levels of both H₂O₂ and salicylic acid gradually declined to the ones before treatment. Moreover, the activities of defense related proteins, including β-1, 3-glucanase, chitinase, and phenylalanine ammonia lyase, significantly increased after lentinan induction, and still kept stable after 96 hours. In addition, the resistance of cucumber treated with lentinan to Colletotrichum orbiculare was notably enhanced after lentinan induction. Both the number and area of lesions on cucumber leaves significantly decreased to 53.1% and 51.3% in comparison with the control, respectively, which indicated that lentinan played a role in inducing the defense responses of cucumber cells.

Key words: lentinan, Cucumis sativus L. cv. Jinfeng, defense response, Colletotrichum orbiculare

ZHANG Wen-hua. The Mechanism of Lentinan Enhancing the Resistance of Cucumber Seedlings to Colletotrichum orbiculare[J]. Biotechnology Bulletin, 2016, 32(3): 93-97.

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