Cloning and Expression Profiles of a Transcription Factor Gene ZjDREB4.1 in Zoysia japonica Under Adversity

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.012

Abstract

Abstract: A novel DREB（dehydration responsive element binding）transcription factor gene,designated as ZjDREB4.1,was isolated from cultivar ‘Meyer’ of Zoysia japonica. The open reading frame（ORF）of ZjDREB4.1 was 651 bp in length,encoding 216 amino acid residues. The putative protein ZjDREB4.1 was 22.9 kD in molecular weight and with a theoretical isoelectric point（pI）of 5.74. Amino acids from the 50^th to the 110^th were predicted to build up a conserved AP2 domain. The phylogenetic tree analysis showed that ZjDREB4.1 was grouped with AtTINY of Arabidopsis thaliana and ZmDBF2 of maize,belonging to the A-4 group of DREB subfamily. ZjDREB4.1 was expressed constitutively in leaves. The expression was up-regulated under cold stress,and was first down-regulated then returned to normal level under drought and salt stresses.

Key words: transcription factor, DREB, semi-quantitative PCR, abiotic stress

LI Jing, WU Qi, ZHANG Lin-jie, LI Xu-ting, ZHOU Min-qi, WEI Shan-jun. Cloning and Expression Profiles of a Transcription Factor Gene ZjDREB4.1 in Zoysia japonica Under Adversity[J]. Biotechnology Bulletin, 2017, 33(2): 80-88.

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