Expression Analysis of Proteasome Subunit PSMD7 After Flagellar Disassembly of Dunaliella salina

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.014

Abstract

Abstract: To study the relationship between the 19S proteasome subunit PSMD7 and flagellar disassembly in Dunaliella salina,the flagellar disassembly was induced by 3-isobutyl-1-methylxanthine（IBMX）and the mRNA expression of PSMD7 was detected by real-time PCR. The high-purity PSMD7 protein of D. salina was obtained by prokaryotic expression and protein purification,followed by the preparation of goat anti-PSMD7 polyclonal antibody. The expression of PSMD7 protein after flagellar disassembly was investigated by Western blot. Results showed that the mRNA expression of PSMD7 increased after flagellar disassembly induced by IBMX and reached the highest at 30 min after disassembly. The goat anti-PSMD7 polyclonal antibody was successfully prepared. Results of Western blot showed that the protein level of PSMD7 rose after flagellar disassembly,indicating that the proteasome subunit PSMD7 was involved in flagellar disassembly of D. salina,thus this provides the basis for further study of the molecular mechanism of ubiquitin-proteasome system in flagellar disassembly.

Key words: PSMD7, proteasome, flagellar disassembly

SHI Ke, LIANG Rui-feng, YANG Liang. Expression Analysis of Proteasome Subunit PSMD7 After Flagellar Disassembly of Dunaliella salina[J]. Biotechnology Bulletin, 2017, 33(3): 93-99.

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