Studies on the Interaction Between FD and 14-3-3/GRF7 in Arabidopsis thaliana

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.017

Abstract

Abstract: In order to prove whether or not there is any interaction between FD and 14-3-3/GRF7 of 14-3-3/GRFs family,both yeast two hybrid and BiFC assays were performed. In yeast,the yeast colonies co-transformed with FD-BD and 14-3-3/GRF7-AD constructs grew well in -Leu/-Trp/-His/-Ade medium comparing with negative controls,indicating that FD directly interacted with 14-3-3/GRF7 in yeast. In tobacco,comparing with negative controls,the obvious YFP fluorescence signals were detected in the tobacco cells in which Agrobacterium co-transformed with FD-cYFP and 14-3-3/GRF7-nYFP,or FD-nYFP and 14-3-3/GRF7-cYFP vectors was injected,revealing that FD also directly interacted with 14-3-3/GRF7 in tobacco. The results by both assays confirm that there is direct interaction between FD and 14-3-3/GRF7 of 14-3-3/GRFs family.

Key words: FD, 14-3-3/GRF7, yeast two hybrid, BiFC

YUAN Min WANG Li GE Wei-na ZHANG Lan. Studies on the Interaction Between FD and 14-3-3/GRF7 in Arabidopsis thaliana[J]. Biotechnology Bulletin, 2017, 33(5): 117-122.

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