Effects of Un-deproteinized Crude Polysaccharides from Cultivated Artemisia rupestris in Xinjiang on Antibody Level and T Lymphocyte Subsets

doi:10.13560/j.cnki.biotech.bull.1985.2016-0964

Abstract

Abstract: This work aims to investigate the effect of un-deproteinized crude polysaccharides（UCARCP）from cultivated Artemisia rupestris L. on antibody level and T lymphocyte subsets in mice immunized ovalbumin（OVA）antigen. Mice were immunized subcutaneously with OVA formulated with UCARCP,priming one time,boosting one time,and aluminum adjuvant for the positive control group. Indirect ELISA assay was performed to evaluate IgG antibody levels,and CD3⁺CD4⁺and CD3⁺CD8⁺T lymphocyte subsets in splenocytes were determined by flow cytometry. As results,UCARCP significantly increased the antibody levels of IgG,IgG₁ and IgG_2a in mice serum（P < 0.05）;and in contrast to aluminum adjuvant group,there was no significant difference（P > 0.05）. Moreover,UCARCP obviously enhanced the percentages of CD3⁺CD4⁺ and CD3⁺CD8⁺T cells（P < 0.05）,and there was also no significant difference compared with that in aluminum adjuvant group（P >0.05）. In conclusion,UCARCP can significantly enhance the humoral and cellular immunity in mice immunized with OVA,and it has the same effects with aluminum adjuvant.

Key words: cultivated Artemisia rupestris L., OVA, antibody, T lymphocyte

WANG Dan-yang, ZHAO Shu-shu, KANG Ya-ling, LUO Xiao-long, TAN Ya-chao, ZHANG Ai-lian, ZHANG Fu-chun. Effects of Un-deproteinized Crude Polysaccharides from Cultivated Artemisia rupestris in Xinjiang on Antibody Level and T Lymphocyte Subsets[J]. Biotechnology Bulletin, 2017, 33(6): 170-175.

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